5HD2

The crystal structure of SeMet-Cry51Aa2-L11M

5HD2 の概要

• エントリーDOI: 10.2210/pdb5hd2/pdb
• 分子名称: Parasporal crystal protein (2 entities in total)
• 機能のキーワード: toxin, cry51aa2, bt, cry
• 由来する生物種: Bacillus thuringiensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34356.02

構造登録者

Rydel, T.J.,Sturman, E.J.,Moshiri, F. (登録日: 2016-01-04, 公開日: 2016-07-20, 最終更新日: 2024-10-30)

主引用文献

Gowda, A.,Rydel, T.J.,Wollacott, A.M.,Brown, R.S.,Akbar, W.,Clark, T.L.,Flasinski, S.,Nageotte, J.R.,Read, A.C.,Shi, X.,Werner, B.J.,Pleau, M.J.,Baum, J.A.
A transgenic approach for controlling Lygus in cotton.
Nat Commun, 7:12213-12213, 2016

PubMed Abstract: Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.

PubMed: 27426014
DOI: 10.1038/ncomms12213

実験手法

X-RAY DIFFRACTION (2.276 Å)