5H6Z

Crystal structure of Set7, a novel histone methyltransferase in Schizossacharomyces pombe

5H6Z の概要

• エントリーDOI: 10.2210/pdb5h6z/pdb
• 分子名称: SET domain-containing protein 7, SULFATE ION, AMMONIUM ION, ... (5 entities in total)
• 機能のキーワード: histone, methytransferase, schizosaccharomyces pombe set7, epigenetic, transcription
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39716.20

構造登録者

Mevius, D.E.H.F.,Shen, Y.,Morishita, M.,Carrozzini, B.,Caliandro, R.,di Luccio, E. (登録日: 2016-11-15, 公開日: 2017-11-22, 最終更新日: 2023-11-08)

主引用文献

Shen, Y.,Mevius, D.E.H.F.,Caliandro, R.,Carrozzini, B.,Roh, Y.,Kim, J.,Kim, S.,Ha, S.C.,Morishita, M.,di Luccio, E.
Set7 Is a H3K37 Methyltransferase in Schizosaccharomyces pombe and Is Required for Proper Gametogenesis.
Structure, 27:631-638.e8, 2019

PubMed Abstract: Histone methylation by histone methyltransferases (HMTases) has a key role in transcriptional regulation. Discrepancies between the known HMTases and the histone lysine methylome suggest that HMTases remain to be identified. Here we report the discovery, characterization, and crystal structure of Schizosaccharomyces pombe Set7, an HMTase methylating the uncharted histone H3 lysine 37 (H3K37) mark. Set7 forms a dimer with its substrate-binding site structurally specific to K37, not the neighboring well-studied K36 mark. We also discovered that H3K37 methylation levels dramatically increase during gametogenesis. Set7 deletion mutant cells show defects in gametogenesis and produce the abnormal number of spores with aberrant morphology. S. pombe gametogenesis shares similarities with mammalian spermatogenesis. These findings extend our understanding of epigenetic regulation during gametogenesis and support a link between Set7, the epigenetic H3K37 methyl mark, and proper gametogenesis.

PubMed: 30773398
DOI: 10.1016/j.str.2019.01.011

実験手法

X-RAY DIFFRACTION (2 Å)