5H47

Crystal structure of AOL complexed with 2-MeSe-Fuc

5H47 の概要

• エントリーDOI: 10.2210/pdb5h47/pdb
• 関連するPDBエントリー: 5EO7 5EO8
• 分子名称: Uncharacterized protein, methyl 6-deoxy-2-Se-methyl-2-seleno-alpha-L-galactopyranoside (3 entities in total)
• 機能のキーワード: seleno-fucoses, phaing, lectin, aspergillus oryzae, sugar binding protein
• 由来する生物種: Aspergillus oryzae RIB40 (Yellow koji mold)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 433279.84

構造登録者

Kato, R.,Nishikawa, Y.,Makyio, H. (登録日: 2016-10-31, 公開日: 2017-01-25, 最終更新日: 2024-03-20)

主引用文献

Shimabukuro, J.,Makyio, H.,Suzuki, T.,Nishikawa, Y.,Kawasaki, M.,Imamura, A.,Ishida, H.,Ando, H.,Kato, R.,Kiso, M.
Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing
Bioorg. Med. Chem., 25:1132-1142, 2017

PubMed Abstract: Selenium-incorporated fucoses (seleno-fucoses) differing in the position of the seleno-substituent were synthesized and applied to the X-ray structural determination of a carbohydrate-lectin complex using single/multi-wavelength anomalous dispersion (SAD/MAD) phasing. The hydroxyl groups at the C-1, -2, -3 and -4 position of fucose were individually substituted with a methylseleno group via a transacetalization reaction using MeSeCHOBn or by an S2 reaction with TolSe equivalents to afford the corresponding MeSe-fucose. The three-dimensional structures of a fucose-binding lectin complexed with several of these MeSe-fucoses have been determined by SAD/MAD phasing by utilizing the diffraction of selenium in the bound MeSe-fucoses.

PubMed: 28041800
DOI: 10.1016/j.bmc.2016.12.021

実験手法

X-RAY DIFFRACTION (2.3 Å)