5H3N

Solution structure of human Gelsolin protein domain 1 at pH 7.3

5H3N の概要

• エントリーDOI: 10.2210/pdb5h3n/pdb
• 関連するPDBエントリー: 5H3M
• NMR情報: BMRB: 36027
• 分子名称: Gelsolin (1 entity in total)
• 機能のキーワード: gelsolin, structural protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14973.82

構造登録者

Fan, J.S.,Yang, D. (登録日: 2016-10-26, 公開日: 2017-11-01, 最終更新日: 2024-05-15)

主引用文献

Fan, J.S.,Goh, H.,Ding, K.,Xue, B.,Robinson, R.C.,Yang, D.
Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1.
Sci Rep, 7:45230-45230, 2017

PubMed Abstract: Six-domain gelsolin regulates actin structural dynamics through its abilities to sever, cap and uncap F-actin. These activities are modulated by various cellular parameters like Ca and pH. Until now, only the molecular activation mechanism of gelsolin by Ca has been understood relatively well. The fragment comprising the first domain and six residues from the linker region into the second domain has been shown to be similar to the full-length protein in F-actin severing activity in the absence of Ca at pH 5. To understand how this gelsolin fragment is activated for F-actin severing by lowering pH, we solved its NMR structures at both pH 7.3 and 5 in the absence of Ca and measured the pKa values of acidic amino acid residues and histidine residues. The overall structure and dynamics of the fragment are not affected significantly by pH. Nevertheless, local structural changes caused by protonation of His29 and Asp109 result in the activation on lowering the pH, and protonation of His151 directly effects filament binding since it resides in the gelsolin/actin interface. Mutagenesis studies support that His29, Asp109 and His151 play important roles in the pH-dependent severing activity of the gelsolin fragment.

PubMed: 28349924
DOI: 10.1038/srep45230

実験手法

SOLUTION NMR