5H2B

Structure of a novel antibody G196

5H2B の概要

• エントリーDOI: 10.2210/pdb5h2b/pdb
• 分子名称: G196 antibody Heavy chain, G196 antibody Light chain (3 entities in total)
• 機能のキーワード: antibody, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47263.56

構造登録者

Park, S.Y.,Sugiyama, K. (登録日: 2016-10-14, 公開日: 2017-03-22, 最終更新日: 2024-10-16)

主引用文献

Tatsumi, K.,Sakashita, G.,Nariai, Y.,Okazaki, K.,Kato, H.,Obayashi, E.,Yoshida, H.,Sugiyama, K.,Park, S.Y.,Sekine, J.,Urano, T.
G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity.
Sci Rep, 7:43480-43480, 2017

PubMed Abstract: The recognition specificity of monoclonal antibodies (mAbs) has made mAbs among the most frequently used tools in both basic science research and in clinical diagnosis and therapies. Precise determination of the epitope allows the development of epitope tag systems to be used with recombinant proteins for various purposes. Here we describe a new family of tag derived from the epitope recognized by a highly specific mAb G196. The minimal epitope was identified as the five amino acid sequence Asp-Leu-Val-Pro-Arg. Permutation analysis was used to characterize the binding requirements of mAb G196, and the variable regions of the mAb G196 were identified and structurally analyzed by X-ray crystallography. Isothermal titration calorimetry revealed the high affinity (K = 1.25 nM) of the mAb G196/G196-epitope peptide interaction, and G196-tag was used to detect several recombinant cytosolic and nuclear proteins in human and yeast cells. mAb G196 is valuable for developing a new peptide tagging system for cell biology and biochemistry research.

PubMed: 28266535
DOI: 10.1038/srep43480

実験手法

X-RAY DIFFRACTION (2.001 Å)