5H18

Crystal structure of catalytic domain of UGGT (UDP-glucose-bound form) from Thermomyces dupontii

5H18 の概要

• エントリーDOI: 10.2210/pdb5h18/pdb
• 分子名称: UGGT, URIDINE-5'-DIPHOSPHATE-GLUCOSE, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: endoplasmic reticulum, quality control, glucosyltransferase, folding sensor, transferase
• 由来する生物種: Thermomyces dupontii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35796.09

構造登録者

Satoh, T.,Zhu, T.,Toshimori, T.,Kamikubo, H.,Uchihashi, T.,Kato, K. (登録日: 2016-10-08, 公開日: 2017-09-27, 最終更新日: 2024-10-23)

主引用文献

Satoh, T.,Song, C.,Zhu, T.,Toshimori, T.,Murata, K.,Hayashi, Y.,Kamikubo, H.,Uchihashi, T.,Kato, K.
Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT.
Sci Rep, 7:12142-12142, 2017

PubMed Abstract: In the endoplasmic reticulum (ER), a protein quality control system facilitates the efficient folding of newly synthesised proteins. In this system, a series of N-linked glycan intermediates displayed on the protein surface serve as quality tags. The ER folding-sensor enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) acts as a gatekeeper in the ER quality control system by specifically catalysing monoglucosylation onto incompletely folded glycoproteins, thereby enabling them to interact with lectin-chaperone complexes. Here we characterise the dynamic structure of this enzyme. Our crystallographic data demonstrate that the sensor region is composed of four thioredoxin-like domains followed by a β-rich domain, which are arranged into a C-shaped structure with a large central cavity, while the C-terminal catalytic domain undergoes a ligand-dependent conformational alteration. Furthermore, small-angle X-ray scattering, cryo-electron microscopy and high-speed atomic force microscopy have demonstrated that UGGT has a flexible modular structure in which the smaller catalytic domain is tethered to the larger folding-sensor region with variable spatial arrangements. These findings provide structural insights into the working mechanism whereby UGGT operates as a folding-sensor against a variety of glycoprotein substrates through its flexible modular structure possessing extended hydrophobic surfaces for the recognition of unfolded substrates.

PubMed: 28939828
DOI: 10.1038/s41598-017-12283-w

実験手法

X-RAY DIFFRACTION (1.4 Å)