5GT5

Structural basis of the specific activity and thermostability of pectate lyase (pelN) from Paenibacillus sp. 0602

5GT5 の概要

• エントリーDOI: 10.2210/pdb5gt5/pdb
• 分子名称: Pectate lyase (2 entities in total)
• 機能のキーワード: pectate lyase, paenibacillus sp. 0602, lyase
• 由来する生物種: Paenibacillus sp. 0602
• 細胞内の位置: Secreted : W8CR80
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96547.01

構造登録者

Zhou, Z.P.,Liu, Y.,Song, J.N. (登録日: 2016-08-18, 公開日: 2017-10-25, 最終更新日: 2023-11-08)

主引用文献

Zhou, Z.,Liu, Y.,Chang, Z.,Wang, H.,Leier, A.,Marquez-Lago, T.T.,Ma, Y.,Li, J.,Song, J.
Structure-based engineering of a pectate lyase with improved specific activity for ramie degumming.
Appl. Microbiol. Biotechnol., 101:2919-2929, 2017

PubMed Abstract: Biotechnological applications of microbial pectate lyases (Pels) in plant fiber processing are promising, eco-friendly substitutes for conventional chemical degumming processes. However, to potentiate the enzymes' use for industrial applications, resolving the molecular structure to elucidate catalytic mechanisms becomes necessary. In this manuscript, we report the high resolution (1.45 Å) crystal structure of pectate lyase (pelN) from Paenibacillus sp. 0602 in apo form. Through sequence alignment and structural superposition with other members of the polysaccharide lyase (PL) family 1 (PL1), we determined that pelN shares the characteristic right-handed β-helix and is structurally similar to other members of the PL1 family, while exhibiting key differences in terms of catalytic and substrate binding residues. Then, based on information from structure alignments with other PLs, we engineered a novel pelN. Our rational design yielded a pelN mutant with a temperature for enzymatic activity optimally shifted from 67.5 to 60 °C. Most importantly, this pelN mutant displayed both higher specific activity and ramie fiber degumming ability when compared with the wild-type enzyme. Altogether, our rational design method shows great potential for industrial applications. Moreover, we expect the reported high-resolution crystal structure to provide a solid foundation for future rational, structure-based engineering of genetically enhanced pelNs.

PubMed: 28028551
DOI: 10.1007/s00253-016-7994-6

実験手法

X-RAY DIFFRACTION (1.449 Å)