5GSS

HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH GLUTATHIONE

Summary for 5GSS

• Entry DOI: 10.2210/pdb5gss/pdb
• Descriptor: GLUTATHIONE S-TRANSFERASE P1-1, GLUTATHIONE, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• Functional Keywords: transferase, detoxifying enzyme
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 47693.50

Authors

Oakley, A.,Parker, M. (deposition date: 1997-08-11, release date: 1998-09-16, Last modification date: 2024-05-22)

Primary citation

Oakley, A.J.,Bello, M.L.,Battistoni, A.,Ricci, G.,Rossjohn, J.,Villar, H.O.,Parker, M.W.
The structures of human glutathione transferase P1-1 in complex with glutathione and various inhibitors at high resolution.
J.Mol.Biol., 274:84-100, 1997

PubMed Abstract: The human pi-class glutathione S-transferase (hGST P1-1) is a target for structure-based inhibitor design with the aim of developing drugs that could be used as adjuvants in chemotherapeutic treatment. Here we present seven crystal structures of the enzyme in complex with substrate (glutathione) and two inhibitors (S-hexyl glutathione and gamma-glutamyl- (S-benzyl)cysteinyl-D-phenylglycine). The binding of the modified glutathione inhibitor, gamma-glutamyl-(S-benzyl)cysteinyl-D-phenylglycine, has been characterized with the phenyl group stacking against the benzyl moiety of the inhibitor and making interactions with the active-site residues Phe8 and Trp38. The structure provides an explanation as to why this compound inhibits the pi-class GST much better than the other GST classes. The structure of the enzyme in complex with glutathione has been determined to high resolution (1.9 to 2.2 A) in three different crystal forms and at two different temperatures (100 and 288 K). In one crystal form, the direct hydrogen-bonding interaction between the hydroxyl group of Tyr7, a residue involved in catalysis, and the thiol group of the substrate, glutathione, is broken and replaced by a water molecule that mediates the interaction. The hydrogen-bonding partner of the hydroxyl group of Tyr108, another residue implicated in the catalysis, is space-group dependent. A high-resolution (2.0 A) structure of the enzyme in complex with S-hexyl glutathione in a new crystal form is presented. The enzyme-inhibitor complexes show that the binding of ligand into the electrophilic binding site does not lead to any conformational changes of the protein.

PubMed: 9398518
DOI: 10.1006/jmbi.1997.1364

Experimental method

X-RAY DIFFRACTION (1.95 Å)