5GPI

Crystal Structures of Unlinked NS2B-NS3 Protease from Zika Virus and Its Complex with a Reverse Peptide Inhibitor

5GPI の概要

• エントリーDOI: 10.2210/pdb5gpi/pdb
• 分子名称: NS3 protease, NS2B cofactor (3 entities in total)
• 機能のキーワード: zika virus protease, serine protease, non-structural protein 3, viral protein
• 由来する生物種: Zika virus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 99611.90

構造登録者

Phoo, W.W.,Zhang, Z.Z. (登録日: 2016-08-02, 公開日: 2016-12-14, 最終更新日: 2023-11-08)

主引用文献

Zhang, Z.,Li, Y.,Loh, Y.R.,Phoo, W.W.,Hung, A.W.,Kang, C.,Luo, D.
Crystal structure of unlinked NS2B-NS3 protease from Zika virus
Science, 354:1597-1600, 2016

PubMed Abstract: Zika virus (ZIKV) has rapidly emerged as a global public health concern. Viral NS2B-NS3 protease processes viral polyprotein and is essential for the virus replication, making it an attractive antiviral drug target. We report crystal structures at 1.58-angstrom resolution of the unlinked NS2B-NS3 protease from ZIKV as free enzyme and bound to a peptide reversely oriented at the active site. The unlinked NS2B-NS3 protease adopts a closed conformation in which NS2B engages NS3 to form an empty substrate-binding site. A second protease in the same crystal binds to the residues K14K15G16E17 from the neighboring NS3 in reverse orientation, resisting proteolysis. These features of ZIKV NS2B-NS3 protease may accelerate the discovery of structure-based antiviral drugs against ZIKV and related pathogenic flaviviruses.

PubMed: 27940580
DOI: 10.1126/science.aai9309

実験手法

X-RAY DIFFRACTION (1.578 Å)