5GP9

Structural analysis of fatty acid degradation regulator FadR from Bacillus halodurans

5GP9 の概要

• エントリーDOI: 10.2210/pdb5gp9/pdb
• 関連するPDBエントリー: 5GPA 5GPC
• 分子名称: Transcriptional regulator (TetR/AcrR family), MAGNESIUM ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: fadr, transcriptional regulator, transcription, dna binding protein
• 由来する生物種: Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45283.18

構造登録者

Lee, J.Y.,Yeo, H.K.,Park, Y.W. (登録日: 2016-08-01, 公開日: 2017-03-01, 最終更新日: 2023-11-08)

主引用文献

Yeo, H.K.,Park, Y.W.,Lee, J.Y.
Structural basis of operator sites recognition and effector binding in the TetR family transcription regulator FadR.
Nucleic Acids Res., 45:4244-4254, 2017

PubMed Abstract: FadR is a fatty acyl-CoA dependent transcription factor that regulates genes encoding proteins involved in fatty-acid degradation and synthesis pathways. In this study, the crystal structures of Bacillus halodurans FadR, which belong to the TetR family, have been determined in three different forms: ligand-bound, ligand-free and DNA-bound at resolutions of 1.75, 2.05 and 2.80 Å, respectively. Structural and functional data showed that B. halodurans FadR was bound to its operator site without fatty acyl-CoAs. Structural comparisons among the three different forms of B. halodurans FadR revealed that the movement of DNA binding domains toward the operator DNA was blocked upon binding of ligand molecules. These findings suggest that the TetR family FadR negatively regulates the genes involved in fatty acid metabolism by binding cooperatively to the operator DNA as a dimer of dimers.

PubMed: 28160603
DOI: 10.1093/nar/gkx009

実験手法

X-RAY DIFFRACTION (1.755 Å)