5GMK

Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution

5GMK の概要

• エントリーDOI: 10.2210/pdb5gmk/pdb
• EMDBエントリー: 9525 9526 9527
• 分子名称: Pre-mRNA-splicing factor 8, Pre-mRNA-splicing factor PRP46, Pre-mRNA-processing protein 45, ... (38 entities in total)
• 機能のキーワード: rna splicing, spliceosome, catalytic step i, intron lariat, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 2023545.51

構造登録者

Wan, R.,Yan, C.,Bai, R.,Huang, G.,Shi, Y. (登録日: 2016-07-14, 公開日: 2016-08-17, 最終更新日: 2024-03-27)

主引用文献

Wan, R.,Yan, C.,Bai, R.,Huang, G.,Shi, Y.
Structure of a yeast catalytic step I spliceosome at 3.4 angstrom resolution
Science, 353:895-904, 2016

PubMed Abstract: Each cycle of pre-messenger RNA splicing, carried out by the spliceosome, comprises two sequential transesterification reactions, which result in the removal of an intron and the joining of two exons. Here we report an atomic structure of a catalytic step I spliceosome (known as the C complex) from Saccharomyces cerevisiae, as determined by cryo-electron microscopy at an average resolution of 3.4 angstroms. In the structure, the 2'-OH of the invariant adenine nucleotide in the branch point sequence (BPS) is covalently joined to the phosphate at the 5' end of the 5' splice site (5'SS), forming an intron lariat. The freed 5' exon remains anchored to loop I of U5 small nuclear RNA (snRNA), and the 5'SS and BPS of the intron form duplexes with conserved U6 and U2 snRNA sequences, respectively. Specific placement of these RNA elements at the catalytic cavity of Prp8 is stabilized by 15 protein components, including Snu114 and the splicing factors Cwc21, Cwc22, Cwc25, and Yju2. These features, representing the conformation of the spliceosome after the first-step reaction, predict structural changes that are needed for the execution of the second-step transesterification reaction.

PubMed: 27445308
DOI: 10.1126/science.aag2235

実験手法

ELECTRON MICROSCOPY (3.4 Å)