5GHK

Crystal Structure Analysis of Canine serum albumin

5GHK の概要

• エントリーDOI: 10.2210/pdb5ghk/pdb
• 分子名称: Serum albumin (1 entity in total)
• 機能のキーワード: plasma protein, transport protein
• 由来する生物種: Canis lupus familiaris (Dog)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65801.04

構造登録者

Kihira, K.,Yamada, K.,Kureishi, M.,Yokomaku, K.,Shinohara, R.,Akiyama, M.,Komatsu, T. (登録日: 2016-06-20, 公開日: 2016-11-23, 最終更新日: 2024-10-16)

主引用文献

Yamada, K.,Yokomaku, K.,Kureishi, M.,Akiyama, M.,Kihira, K.,Komatsu, T.
Artificial Blood for Dogs
Sci Rep, 6:36782-36782, 2016

PubMed Abstract: There is no blood bank for pet animals. Consequently, veterinarians themselves must obtain "blood" for transfusion therapy. Among the blood components, serum albumin and red blood cells (RBCs) are particularly important to save lives. This paper reports the synthesis, structure, and properties of artificial blood for the exclusive use of dogs. First, recombinant canine serum albumin (rCSA) was produced using genetic engineering with Pichia yeast. The proteins showed identical features to those of the native CSA derived from canine plasma. Furthermore, we ascertained the crystal structure of rCSA at 3.2 Å resolution. Pure rCSA can be used widely for numerous clinical and pharmaceutical applications. Second, hemoglobin wrapped covalently with rCSA, hemoglobin-albumin cluster (Hb-rCSA), was synthesized as an artificial O-carrier for the RBC substitute. This cluster possesses satisfactorily negative surface net charge (pI = 4.7), which supports enfolding of the Hb core by rCSA shells. The anti-CSA antibody recognized the rCSA exterior quantitatively. The O-binding affinity was high (P = 9 Torr) compared to that of the native Hb. The Hb-rCSA cluster is anticipated for use as an alternative material for RBC transfusion, and as an O therapeutic reagent that can be exploited in various veterinary medicine situations.

PubMed: 27830776
DOI: 10.1038/srep36782

実験手法

X-RAY DIFFRACTION (3.2 Å)