5G61

S.pneumoniae ABC-transporter substrate binding protein FusA in complex with fructo-nystose

5G61 の概要

• エントリーDOI: 10.2210/pdb5g61/pdb
• 関連するPDBエントリー: 5G5Y 5G5Z 5G60 5G62
• 関連するBIRD辞書のPRD_ID: PRD_900056
• 分子名称: ABC TRANSPORTER, SUBSTRATE-BINDING PROTEIN, beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-fructofuranose-(2-1)-beta-D-sorbofuranose-(2-1)-beta-L-talopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: transport protein, fusa, substrate-binding-protein, abc-tran transporter, fructooligosaccharides, kestose, nystose, fructo-nystose, carbohydrate, sugar, transport, pneumoniae
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 229038.45

構造登録者

Culurgioni, S.,Harris, G.,Singh, A.K.,King, S.J.,Walsh, M.A. (登録日: 2016-06-10, 公開日: 2017-01-18, 最終更新日: 2024-01-10)

主引用文献

Culurgioni, S.,Harris, G.,Singh, A.K.,King, S.J.,Walsh, M.A.
Structural Basis for Regulation and Specificity of Fructooligosaccharide Import in Streptococcus pneumoniae.
Structure, 25:79-93, 2017

PubMed Abstract: Streptococcus pneumoniae is dependent on carbohydrate uptake for colonization and pathogenesis, and dedicates over a third of its transport systems to their uptake. The ability of the pneumococcus to utilize fructooligosaccharides (FOSs) is attributed to the presence of one of two types of FOS ATP-binding cassette (ABC) transporters. Strains encoding SfuABC are only able to utilize short-chain FOSs, while strains encoding FusABC can utilize both short- and long-chain FOSs. The crystal structures of the substrate-binding protein FusA in its open and closed conformations bound to FOSs, and solution scattering data of SfuA, delineate the structural basis for import of short- and long-chain FOSs. The structure of FusA identifies an EF hand-like calcium-binding motif. This is shown to be essential for translocation of FOSs in FusABC and forms the basis for the definition of a new class of substrate-binding proteins that regulate substrate translocation by calcium.

PubMed: 27939783
DOI: 10.1016/j.str.2016.11.008

実験手法

X-RAY DIFFRACTION (2.4 Å)