5G5L

RNA polymerase I-Rrn3 complex at 4.8 A resolution

Summary for 5G5L

• Entry DOI: 10.2210/pdb5g5l/pdb
• EMDB information: 3439
• Descriptor: DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA190, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5, DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2, ... (16 entities in total)
• Functional Keywords: rna polymerase, transciption
• Biological source: SACCHAROMYCES CEREVISIAE (BAKERS YEAST); More
• Total number of polymer chains: 15
• Total formula weight: 663194.65

Authors

Engel, C.,Plitzko, J.,Cramer, P. (deposition date: 2016-05-26, release date: 2016-07-27, Last modification date: 2024-10-09)

Primary citation

Engel, C.,Plitzko, J.,Cramer, P.
RNA Polymerase I-Rrn3 Complex at 4.8 A Resolution
Nat.Commun., 7:12129-, 2016

PubMed Abstract: Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I-Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex and provides insights into the mechanisms of Pol I-specific initiation and regulation.

PubMed: 27418309
DOI: 10.1038/NCOMMS12129

Experimental method

ELECTRON MICROSCOPY (4.8 Å)