5G2X

Structure a of Group II Intron Complexed with its Reverse Transcriptase

5G2X の概要

• エントリーDOI: 10.2210/pdb5g2x/pdb
• 関連するPDBエントリー: 5G2Y
• EMDBエントリー: 3331
• 分子名称: GROUP II INTRON, 5'-R(*CP*AP*CP*AP*UP*CP*CP*AP*UP*AP*AP*CP)-3', GROUP II INTRON-ENCODED PROTEIN LTRA (3 entities in total)
• 機能のキーワード: transferase, group ii introns, ribonucleoprotein, intron-encoded protein, retrotransposons and spliceosom
• 由来する生物種: LACTOCOCCUS LACTIS; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 301653.45

構造登録者

Qu, G.,Kaushal, P.S.,Wang, J.,Shigematsu, H.,Piazza, C.L.,Agrawal, R.K.,Belfort, M.,Wang, H.W. (登録日: 2016-04-16, 公開日: 2016-05-11, 最終更新日: 2024-05-08)

主引用文献

Qu, G.,Kaushal, P.S.,Wang, J.,Shigematsu, H.,Piazza, C.L.,Agrawal, R.K.,Belfort, M.,Wang, H.
Structure of a Group II Intron in Complex with its Reverse Transcriptase.
Nat.Struct.Mol.Biol., 23:549-, 2016

PubMed Abstract: Bacterial group II introns are large catalytic RNAs related to nuclear spliceosomal introns and eukaryotic retrotransposons. They self-splice, yielding mature RNA, and integrate into DNA as retroelements. A fully active group II intron forms a ribonucleoprotein complex comprising the intron ribozyme and an intron-encoded protein that performs multiple activities including reverse transcription, in which intron RNA is copied into the DNA target. Here we report cryo-EM structures of an endogenously spliced Lactococcus lactis group IIA intron in its ribonucleoprotein complex form at 3.8-Å resolution and in its protein-depleted form at 4.5-Å resolution, revealing functional coordination of the intron RNA with the protein. Remarkably, the protein structure reveals a close relationship between the reverse transcriptase catalytic domain and telomerase, whereas the active splicing center resembles the spliceosomal Prp8 protein. These extraordinary similarities hint at intricate ancestral relationships and provide new insights into splicing and retromobility.

PubMed: 27136327
DOI: 10.1038/NSMB.3220

実験手法

ELECTRON MICROSCOPY (3.8 Å)