5FZ5

Transcription initiation complex structures elucidate DNA opening (CC)

5FZ5 の概要

• エントリーDOI: 10.2210/pdb5fz5/pdb
• 関連するPDBエントリー: 5FYW
• EMDBエントリー: 3383
• 分子名称: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5, DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11, ... (24 entities in total)
• 機能のキーワード: transcription, gene expression, transcription initiation
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 890127.28

構造登録者

Plaschka, C.,Hantsche, M.,Dienemann, C.,Burzinski, C.,Plitzko, J.,Cramer, P. (登録日: 2016-03-10, 公開日: 2016-05-18, 最終更新日: 2024-05-08)

主引用文献

Plaschka, C.,Hantsche, M.,Dienemann, C.,Burzinski, C.,Plitzko, J.,Cramer, P.
Transcription Initiation Complex Structures Elucidate DNA Opening
Nature, 533:353-, 2016

PubMed Abstract: Transcription of eukaryotic protein-coding genes begins with assembly of the RNA polymerase (Pol) II initiation complex and promoter DNA opening. Here we report cryo-electron microscopy (cryo-EM) structures of yeast initiation complexes containing closed and open DNA at resolutions of 8.8 Å and 3.6 Å, respectively. DNA is positioned and retained over the Pol II cleft by a network of interactions between the TATA-box-binding protein TBP and transcription factors TFIIA, TFIIB, TFIIE, and TFIIF. DNA opening occurs around the tip of the Pol II clamp and the TFIIE 'extended winged helix' domain, and can occur in the absence of TFIIH. Loading of the DNA template strand into the active centre may be facilitated by movements of obstructing protein elements triggered by allosteric binding of the TFIIE 'E-ribbon' domain. The results suggest a unified model for transcription initiation with a key event, the trapping of open promoter DNA by extended protein-protein and protein-DNA contacts.

PubMed: 27193681
DOI: 10.1038/NATURE17990

実験手法

ELECTRON MICROSCOPY (8.8 Å)