5FX0

Fasciola hepatica calcium binding protein FhCaBP2: Structure of the dynein light chain-like domain. P6422 native.

Summary for 5FX0

• Entry DOI: 10.2210/pdb5fx0/pdb
• Related: 5FWZ
• Descriptor: CALCIUM BINDING PROTEIN (2 entities in total)
• Functional Keywords: cell adhesion
• Biological source: FASCIOLA HEPATICA (LIVER FLUKE)
• Total number of polymer chains: 1
• Total formula weight: 12522.27

Authors

Nguyen, T.H.,Thomas, C.M.,Timson, D.J.,van Raaij, M.J. (deposition date: 2016-02-22, release date: 2016-04-27, Last modification date: 2024-01-10)

Primary citation

Nguyen, T.H.,Thomas, C.M.,Timson, D.J.,van Raaij, M.J.
Fasciola hepatica calcium-binding protein FhCaBP2: structure of the dynein light chain-like domain.
Parasitol. Res., 115:2879-2886, 2016

PubMed Abstract: The common liver fluke Fasciola hepatica causes an increasing burden on human and animal health, partly because of the spread of drug-resistant isolates. As a consequence, there is considerable interest in developing new drugs to combat liver fluke infections. A group of potential targets is a family of calcium-binding proteins which combine an N-terminal domain with two EF-hand motifs and a C-terminal domain with predicted similarity to dynein light chains (DLC-like domain). The function of these proteins is unknown, although in several species, they have been localised to the tegument, an important structure at the host-parasite interface. Here, we report the X-ray crystal structure of the DLC-like domain of F. hepatica calcium-binding protein 2 (FhCaBP2), solved using single-wavelength anomalous diffraction and refined at 2.3 Å resolution in two different crystal forms. The FhCaBP2 DLC-like domain has a structure similar to other DLC domains, with an anti-parallel β-sheet packed against an α-helical hairpin. Like other DLC domains, it dimerises through its β2-strand, which extends in an arch and forms the fifth strand in an extended β-sheet of the other monomer. The structure provides molecular details of the dimerisation of FhCaBP2, the first example from this family of parasite proteins.

PubMed: 27083189
DOI: 10.1007/s00436-016-5046-x

Experimental method

X-RAY DIFFRACTION (2.3 Å)