5FWT

Wnt modulator Kremen crystal form I at 2.10A

5FWT の概要

• エントリーDOI: 10.2210/pdb5fwt/pdb
• 関連するPDBエントリー: 5FWS 5FWU 5FWV 5FWW
• 分子名称: KREMEN PROTEIN 1, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: signaling protein, wnt, cell surface, signalling, membrane protein
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Membrane ; Single-pass type I membrane protein : Q96MU8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45350.45

構造登録者

Zebisch, M.,Jackson, V.A.,Jones, E.Y. (登録日: 2016-02-21, 公開日: 2016-07-20, 最終更新日: 2024-10-09)

主引用文献

Zebisch, M.,Jackson, V.A.,Zhao, Y.,Jones, E.Y.
Structure of the Dual-Mode Wnt Regulator Kremen1 and Insight Into Ternary Complex Formation with Lrp6 and Dickkopf
Structure, 24:1599-, 2016

PubMed Abstract: Kremen 1 and 2 have been identified as co-receptors for Dickkopf (Dkk) proteins, hallmark secreted antagonists of canonical Wnt signaling. We present here three crystal structures of the ectodomain of human Kremen1 (KRM1ECD) at resolutions between 1.9 and 3.2 Å. KRM1ECD emerges as a rigid molecule with tight interactions stabilizing a triangular arrangement of its Kringle, WSC, and CUB structural domains. The structures reveal an unpredicted homology of the WSC domain to hepatocyte growth factor. We further report the general architecture of the ternary complex formed by the Wnt co-receptor Lrp5/6, Dkk, and Krm, determined from a low-resolution complex crystal structure between β-propeller/EGF repeats (PE) 3 and 4 of the Wnt co-receptor LRP6 (LRP6PE3PE4), the cysteine-rich domain 2 (CRD2) of DKK1, and KRM1ECD. DKK1CRD2 is sandwiched between LRP6PE3 and KRM1Kringle-WSC. Modeling studies supported by surface plasmon resonance suggest a direct interaction site between Krm1CUB and Lrp6PE2.

PubMed: 27524201
DOI: 10.1016/J.STR.2016.06.020

実験手法

X-RAY DIFFRACTION (2.1 Å)