5FV6

KpFlo11 presents a novel member of the Flo11 family with a unique recognition pattern for homophilic interactions

5FV6 の概要

• エントリーDOI: 10.2210/pdb5fv6/pdb
• 関連するPDBエントリー: 5FV5
• 分子名称: Flocculation protein FLO11, ACETATE ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: cell adhesion, homophilic cell-cell adhesion, fibronectin type iii domain, fungal cell wall, aromatic surface patches, surface-plasmon resonance spectroscopy, cell adhesio
• 由来する生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast, Pichia pastoris)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44246.96

構造登録者

Kraushaar, T.,Brueckner, S.,Mikolaiski, M.,Schreiner, F.,Veelders, M.,Moesch, H.U.,Essen, L.O. (登録日: 2016-02-03, 公開日: 2017-02-22, 最終更新日: 2024-10-16)

主引用文献

Bruckner, S.,Schubert, R.,Kraushaar, T.,Hartmann, R.,Hoffmann, D.,Jelli, E.,Drescher, K.,Muller, D.J.,Oliver Essen, L.,Mosch, H.U.
Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family.
Elife, 9:-, 2020

PubMed Abstract: Microorganisms have evolved specific cell surface molecules that enable discrimination between cells from the same and from a different kind. Here, we investigate the role of Flo11-type cell surface adhesins from social yeasts in kin discrimination. We measure the adhesion forces mediated by Flo11A-type domains using single-cell force spectroscopy, quantify Flo11A-based cell aggregation in populations and determine the Flo11A-dependent segregation of competing yeast strains in biofilms. We find that Flo11A domains from diverse yeast species confer remarkably strong adhesion forces by establishing homotypic interactions between single cells, leading to efficient cell aggregation and biofilm formation in homogenous populations. Heterotypic interactions between Flo11A domains from different yeast species or strains confer weak adhesive forces and lead to efficient strain segregation in heterogenous populations, indicating that in social yeasts Flo11A-mediated cell adhesion is a major mechanism for kin discrimination at species and sub-species levels. These findings, together with our structure and mutation analysis of selected Flo11A domains, provide a rationale of how cell surface receptors have evolved in microorganisms to mediate kin discrimination.

PubMed: 32286952
DOI: 10.7554/eLife.55587

実験手法

X-RAY DIFFRACTION (2 Å)