5FUU
Ectodomain of cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 Fab
This is a non-PDB format compatible entry.
Summary for 5FUU
| Entry DOI | 10.2210/pdb5fuu/pdb |
| EMDB information | 3308 |
| Descriptor | HIV-1 ENVELOPE GLYCOPROTEIN GP160, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (16 entities in total) |
| Functional Keywords | viral protein, hiv-1, env, pgt151, broadly neutralizing antibody |
| Biological source | HUMAN IMMUNODEFICIENCY VIRUS 1 More |
| Total number of polymer chains | 10 |
| Total formula weight | 353076.17 |
| Authors | Lee, J.H.,Ward, A.B. (deposition date: 2016-01-29, release date: 2016-03-09, Last modification date: 2024-10-23) |
| Primary citation | Lee, J.H.,Ozorowski, G.,Ward, A.B. Cryo-Em Structure of a Native, Fully Glycosylated and Cleaved HIV-1 Envelope Trimer Science, 351:1043-, 2016 Cited by PubMed Abstract: The envelope glycoprotein trimer (Env) on the surface of HIV-1 recognizes CD4(+) T cells and mediates viral entry. During this process, Env undergoes substantial conformational rearrangements, making it difficult to study in its native state. Soluble stabilized trimers have provided valuable insights into the Env structure, but they lack the hydrophobic membrane proximal external region (MPER, an important target of broadly neutralizing antibodies), the transmembrane domain, and the cytoplasmic tail. Here we present (i) a cryogenic electron microscopy (cryo-EM) structure of a clade B virus Env, which lacks only the cytoplasmic tail and is stabilized by the broadly neutralizing antibody PGT151, at a resolution of 4.2 angstroms and (ii) a reconstruction of this form of Env in complex with PGT151 and MPER-targeting antibody 10E8 at a resolution of 8.8 angstroms. These structures provide new insights into the wild-type Env structure. PubMed: 26941313DOI: 10.1126/SCIENCE.AAD2450 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.19 Å) |
Structure validation
Download full validation report
