5FUU

Ectodomain of cleaved wild type JR-FL EnvdCT trimer in complex with PGT151 Fab

これはPDB形式変換不可エントリーです。

5FUU の概要

• エントリーDOI: 10.2210/pdb5fuu/pdb
• EMDBエントリー: 3308
• 分子名称: HIV-1 ENVELOPE GLYCOPROTEIN GP160, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (16 entities in total)
• 機能のキーワード: viral protein, hiv-1, env, pgt151, broadly neutralizing antibody
• 由来する生物種: HUMAN IMMUNODEFICIENCY VIRUS 1; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 353076.17

構造登録者

Lee, J.H.,Ward, A.B. (登録日: 2016-01-29, 公開日: 2016-03-09, 最終更新日: 2024-10-23)

主引用文献

Lee, J.H.,Ozorowski, G.,Ward, A.B.
Cryo-Em Structure of a Native, Fully Glycosylated and Cleaved HIV-1 Envelope Trimer
Science, 351:1043-, 2016

PubMed Abstract: The envelope glycoprotein trimer (Env) on the surface of HIV-1 recognizes CD4(+) T cells and mediates viral entry. During this process, Env undergoes substantial conformational rearrangements, making it difficult to study in its native state. Soluble stabilized trimers have provided valuable insights into the Env structure, but they lack the hydrophobic membrane proximal external region (MPER, an important target of broadly neutralizing antibodies), the transmembrane domain, and the cytoplasmic tail. Here we present (i) a cryogenic electron microscopy (cryo-EM) structure of a clade B virus Env, which lacks only the cytoplasmic tail and is stabilized by the broadly neutralizing antibody PGT151, at a resolution of 4.2 angstroms and (ii) a reconstruction of this form of Env in complex with PGT151 and MPER-targeting antibody 10E8 at a resolution of 8.8 angstroms. These structures provide new insights into the wild-type Env structure.

PubMed: 26941313
DOI: 10.1126/SCIENCE.AAD2450

実験手法

ELECTRON MICROSCOPY (4.19 Å)