5FUA

Cryo-EM of BK polyomavirus

5FUA の概要

• エントリーDOI: 10.2210/pdb5fua/pdb
• EMDBエントリー: 3283
• 分子名称: MAJOR CAPSID PROTEIN VP1 (1 entity in total)
• 機能のキーワード: virus, bkpyv, bk, polyomavirus
• 由来する生物種: BK POLYOMAVIRUS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 240927.70

構造登録者

Hurdiss, D.L.,Morgan, E.L.,Thompson, R.F.,Prescott, E.L.,Panou, M.M.,Macdonald, A.,Ranson, N.A. (登録日: 2016-01-22, 公開日: 2016-04-20, 最終更新日: 2024-05-08)

主引用文献

Hurdiss, D.L.,Morgan, E.L.,Thompson, R.F.,Prescott, E.L.,Panou, M.M.,Macdonald, A.,Ranson, N.A.
New Structural Insights Into the Genome and Minor Capsid Proteins of Bk Polyomavirus Using Cryo-Electron Microscopy.
Structure, 24:528-, 2016

PubMed Abstract: BK polyomavirus is the causative agent of several diseases in transplant patients and the immunosuppressed. In order to better understand the structure and life cycle of BK, we produced infectious virions and VP1-only virus-like particles in cell culture, and determined their three-dimensional structures using cryo-electron microscopy (EM) and single-particle image processing. The resulting 7.6-Å resolution structure of BK and 9.1-Å resolution of the virus-like particles are the highest-resolution cryo-EM structures of any polyomavirus. These structures confirm that the architecture of the major structural protein components of these human polyomaviruses are similar to previous structures from other hosts, but give new insight into the location and role of the enigmatic minor structural proteins, VP2 and VP3. We also observe two shells of electron density, which we attribute to a structurally ordered part of the viral genome, and discrete contacts between this density and both VP1 and the minor capsid proteins.

PubMed: 26996963
DOI: 10.1016/J.STR.2016.02.008

実験手法

ELECTRON MICROSCOPY (7.6 Å)