5FT1

Crystal structure of gp37(Dip) from bacteriophage phiKZ bound to RNase E of Pseudomonas aeruginosa

5FT1 の概要

• エントリーDOI: 10.2210/pdb5ft1/pdb
• 関連するPDBエントリー: 5FT0
• 分子名称: GP37, RIBONUCLEASE E (3 entities in total)
• 機能のキーワード: hydrolase-inhibitor complex, dip, phikz, bacteriophage, rnase e, ribonuclease inhibitor, rna degradosome, pseudomonas aeruginosa, hydrolase/inhibitor
• 由来する生物種: PSEUDOMONAS PHAGE PHIKZ; 詳細
• 細胞内の位置: Cytoplasm : Q9HZM8
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 208172.16

構造登録者

Van den Bossche, A.,Hardwick, S.W.,Ceyssens, P.J.,Hendrix, H.,Voet, M.,Dendooven, T.,Bandyra, K.J.,De Maeyer, M.,Aertsen, A.,Noben, J.P.,Luisi, B.F.,Lavigne, R. (登録日: 2016-01-08, 公開日: 2016-08-03, 最終更新日: 2024-01-10)

主引用文献

Van den Bossche, A.,Hardwick, S.W.,Ceyssens, P.J.,Hendrix, H.,Voet, M.,Dendooven, T.,Bandyra, K.J.,De Maeyer, M.,Aertsen, A.,Noben, J.P.,Luisi, B.F.,Lavigne, R.
Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome.
Elife, 5:-, 2016

PubMed Abstract: In all domains of life, the catalysed degradation of RNA facilitates rapid adaptation to changing environmental conditions, while destruction of foreign RNA is an important mechanism to prevent host infection. We have identified a virus-encoded protein termed gp37/Dip, which directly binds and inhibits the RNA degradation machinery of its bacterial host. Encoded by giant phage фKZ, this protein associates with two RNA binding sites of the RNase E component of the Pseudomonas aeruginosa RNA degradosome, occluding them from substrates and resulting in effective inhibition of RNA degradation and processing. The 2.2 Å crystal structure reveals that this novel homo-dimeric protein has no identifiable structural homologues. Our biochemical data indicate that acidic patches on the convex outer surface bind RNase E. Through the activity of Dip, фKZ has evolved a unique mechanism to down regulate a key metabolic process of its host to allow accumulation of viral RNA in infected cells.

PubMed: 27447594
DOI: 10.7554/eLife.16413

実験手法

X-RAY DIFFRACTION (2.75 Å)