5FRU

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR

5FRU の概要

• エントリーDOI: 10.2210/pdb5fru/pdb
• 関連するPDBエントリー: 5FRV 5FRW 5FRX 5FRY 5FRZ 5FS0
• 分子名称: Positive phenol-degradative gene regulator, ZINC ION (3 entities in total)
• 機能のキーワード: transcription, phenol, poxr, dmpr, enhancer-binding protein, activator, atpase family
• 由来する生物種: Ralstonia sp. E2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47121.01

構造登録者

Patil, V.V.,Woo, E.J. (登録日: 2015-12-23, 公開日: 2016-03-30, 最終更新日: 2024-05-08)

主引用文献

Patil, V.V.,Park, K.-H.,Lee, S.-G.,Woo, E.J.
Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator Poxr
Structure, 624:24-, 2016

PubMed Abstract: Positive phenol-degradative gene regulator (PoxR) is a σ(54)-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering.

PubMed: 27050690
DOI: 10.1016/J.STR.2016.03.006

実験手法

X-RAY DIFFRACTION (1.85 Å)