5FRD

Structure of a thermophilic esterase

5FRD の概要

• エントリーDOI: 10.2210/pdb5frd/pdb
• 分子名称: CARBOXYLESTERASE (EST-2), COENZYME A, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: hydrolase, esterase
• 由来する生物種: ARCHAEOGLOBUS FULGIDUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60628.99

構造登録者

Sayer, C.,Finnigan, W.,Isupov, M.N.,Levisson, M.,Kengen, S.W.M.,van der Oost, J.,Harmer, N.,Littlechild, J.A. (登録日: 2015-12-17, 公開日: 2016-05-25, 最終更新日: 2024-01-10)

主引用文献

Sayer, C.,Finnigan, W.,Isupov, M.N.,Levisson, M.,Kengen, S.W.,Van Der Oost, J.,Harmer, N.J.,Littlechild, J.A.
Structural and Biochemical Characterisation of Archaeoglobus Fulgidus Esterase Reveals a Bound Coa Molecule in the Vicinity of the Active Site.
Sci.Rep., 6:25542-, 2016

PubMed Abstract: A new carboxyl esterase, AF-Est2, from the hyperthermophilic archaeon Archaeoglobus fulgidus has been cloned, over-expressed in Escherichia coli and biochemically and structurally characterized. The enzyme has high activity towards short- to medium-chain p-nitrophenyl carboxylic esters with optimal activity towards the valerate ester. The AF-Est2 has good solvent and pH stability and is very thermostable, showing no loss of activity after incubation for 30 min at 80 °C. The 1.4 Å resolution crystal structure of AF-Est2 reveals Coenzyme A (CoA) bound in the vicinity of the active site. Despite the presence of CoA bound to the AF-Est2 this enzyme has no CoA thioesterase activity. The pantetheine group of CoA partially obstructs the active site alcohol pocket suggesting that this ligand has a role in regulation of the enzyme activity. A comparison with closely related α/β hydrolase fold enzyme structures shows that the AF-Est2 has unique structural features that allow CoA binding. A comparison of the structure of AF-Est2 with the human carboxyl esterase 1, which has CoA thioesterase activity, reveals that CoA is bound to different parts of the core domain in these two enzymes and approaches the active site from opposite directions.

PubMed: 27160974
DOI: 10.1038/SREP25542

実験手法

X-RAY DIFFRACTION (1.4 Å)