5FQ1

Structure of the cytoplasmic PAS domain of the Geobacillus thermodenitrificans histidine kinase CitA

5FQ1 の概要

• エントリーDOI: 10.2210/pdb5fq1/pdb
• 分子名称: HISTIDINE KINASE, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: transferase, pas domain, cita, transmembrane signaling
• 由来する生物種: GEOBACILLUS THERMODENITRIFICANS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25543.12

構造登録者

Schomburg, B.,Giller, K.,Becker, S. (登録日: 2015-12-03, 公開日: 2017-01-11, 最終更新日: 2024-11-20)

主引用文献

Weisenburger, S.,Boening, D.,Schomburg, B.,Giller, K.,Becker, S.,Griesinger, C.,Sandoghdar, V.
Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.
Nat. Methods, 14:141-144, 2017

PubMed Abstract: We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.

PubMed: 28068317
DOI: 10.1038/nmeth.4141

実験手法

X-RAY DIFFRACTION (1.76 Å)