5FPZ

The structure of KdgF from Yersinia enterocolitica with malonate bound in the active site.

5FPZ の概要

• エントリーDOI: 10.2210/pdb5fpz/pdb
• 関連するPDBエントリー: 5FPX 5FQ0
• 分子名称: PECTIN DEGRADATION PROTEIN, NICKEL (II) ION, MALONIC ACID, ... (4 entities in total)
• 機能のキーワード: hydrolase, kdgf, pectin, alginate, uronate sugar metabolism, cupin
• 由来する生物種: YERSINIA ENTEROCOLITICA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12648.93

構造登録者

Hobbs, J.K.,Lee, S.M.,Robb, M.,Hof, F.,Barr, C.,Abe, K.T.,Hehemann, J.H.,McLean, R.,Abbott, D.W.,Boraston, A.B. (登録日: 2015-12-03, 公開日: 2016-05-04, 最終更新日: 2024-01-10)

主引用文献

Hobbs, J.K.,Lee, S.M.,Robb, M.,Hof, F.,Barr, C.,Abe, K.T.,Hehemann, J.,Mclean, R.,Abbott, D.W.,Boraston, A.B.
Kdgf, the Missing Link in the Microbial Metabolism of Uronate Sugars from Pectin and Alginate.
Proc.Natl.Acad.Sci.USA, 113:6188-, 2016

PubMed Abstract: Uronates are charged sugars that form the basis of two abundant sources of biomass-pectin and alginate-found in the cell walls of terrestrial plants and marine algae, respectively. These polysaccharides represent an important source of carbon to those organisms with the machinery to degrade them. The microbial pathways of pectin and alginate metabolism are well studied and essentially parallel; in both cases, unsaturated monouronates are produced and processed into the key metabolite 2-keto-3-deoxygluconate (KDG). The enzymes required to catalyze each step have been identified within pectinolytic and alginolytic microbes; yet the function of a small ORF, kdgF, which cooccurs with the genes for these enzymes, is unknown. Here we show that KdgF catalyzes the conversion of pectin- and alginate-derived 4,5-unsaturated monouronates to linear ketonized forms, a step in uronate metabolism that was previously thought to occur spontaneously. Using enzyme assays, NMR, mutagenesis, and deletion of kdgF, we show that KdgF proteins from both pectinolytic and alginolytic bacteria catalyze the ketonization of unsaturated monouronates and contribute to efficient production of KDG. We also report the X-ray crystal structures of two KdgF proteins and propose a mechanism for catalysis. The discovery of the function of KdgF fills a 50-y-old gap in the knowledge of uronate metabolism. Our findings have implications not only for the understanding of an important metabolic pathway, but also the role of pectinolysis in plant-pathogen virulence and the growing interest in the use of pectin and alginate as feedstocks for biofuel production.

PubMed: 27185956
DOI: 10.1073/PNAS.1524214113

実験手法

X-RAY DIFFRACTION (1.5 Å)