5FOY

De novo structure of the binary mosquito larvicide BinAB at pH 7

5FOY の概要

• エントリーDOI: 10.2210/pdb5foy/pdb
• 関連するPDBエントリー: 5FOZ
• 分子名称: 41.9 KDA INSECTICIDAL TOXIN, LARVICIDAL TOXIN 51 KDA PROTEIN (3 entities in total)
• 機能のキーワード: toxin, binab insecticidal toxin, pore forming toxin, xfel, serial femtosecond crystallography, de novo miras phasing, in vivo crystals
• 由来する生物種: LYSINIBACILLUS SPHAERICUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93442.48

構造登録者

Colletier, J.P.,Sawaya, M.R.,Gingery, M.,Rodriguez, J.A.,Cascio, D.,Brewster, A.S.,Michels-Clark, T.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,DePonte, D.P.,Sierra, R.G.,Laksmono, H.,Koglin, J.E.,Hunter, M.S.,W Park, H.,Uervirojnangkoorn, M.,Bideshi, D.L.,Brunger, A.T.,Federici, B.A.,Sauter, N.K.,Eisenberg, D.S. (登録日: 2015-11-26, 公開日: 2016-10-05, 最終更新日: 2024-11-13)

主引用文献

Colletier, J.,Sawaya, M.R.,Gingery, M.,Rodriguez, J.A.,Cascio, D.,Brewster, A.S.,Michels-Clark, T.,Hice, R.H.,Coquelle, N.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,Deponte, D.P.,Sierra, R.G.,Laksmono, H.,Koglin, J.E.,Hunter, M.S.,Park, H.,Uervirojnangkoorn, M.,Bideshi, D.K.,Brunger, A.T.,Federici, B.A.,Sauter, N.K.,Eisenberg, D.S.
De Novo Phasing with X-Ray Laser Reveals Mosquito Larvicide Binab Structure.
Nature, 539:43-, 2016

PubMed Abstract: BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which play distinct roles in the multi-step intoxication process, transforming from harmless, robust crystals, to soluble protoxin heterodimers, to internalized mature toxin, and finally to toxic oligomeric pores. The small size of the crystals-50 unit cells per edge, on average-has impeded structural characterization by conventional means. Here we report the structure of Lysinibacillus sphaericus BinAB solved de novo by serial-femtosecond crystallography at an X-ray free-electron laser. The structure reveals tyrosine- and carboxylate-mediated contacts acting as pH switches to release soluble protoxin in the alkaline larval midgut. An enormous heterodimeric interface appears to be responsible for anchoring BinA to receptor-bound BinB for co-internalization. Remarkably, this interface is largely composed of propeptides, suggesting that proteolytic maturation would trigger dissociation of the heterodimer and progression to pore formation.

PubMed: 27680699
DOI: 10.1038/NATURE19825

実験手法

X-RAY DIFFRACTION (2.25 Å)