5FO9

Crystal Structure of Human Complement C3b in Complex with CR1 (CCP15- 17)

5FO9 の概要

• エントリーDOI: 10.2210/pdb5fo9/pdb
• 関連するPDBエントリー: 5FO7 5FO8 5FOA 5FOB
• 分子名称: COMPLEMENT C3 BETA CHAIN, COMPLEMENT C3B ALPHA' CHAIN, COMPLEMENT RECEPTOR TYPE 1, ... (4 entities in total)
• 機能のキーワード: lipid binding protein, complement system, immune system, plasma protein, regulators of complement activity, cofactor activity, decay accelerating activity
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 394612.41

構造登録者

Forneris, F.,Wu, J.,Xue, X.,Gros, P. (登録日: 2015-11-18, 公開日: 2016-04-06, 最終更新日: 2025-10-01)

主引用文献

Forneris, F.,Wu, J.,Xue, X.,Ricklin, D.,Lin, Z.,Sfyroera, G.,Tzekou, A.,Volokhina, E.,Granneman, J.C.,Hauhart, R.,Bertram, P.,Liszewski, M.K.,Atkinson, J.P.,Lambris, J.D.,Gros, P.
Regulators of Complement Activity Mediate Inhibitory Mechanisms Through a Common C3B-Binding Mode.
Embo J., 35:1133-, 2016

PubMed Abstract: Regulators of complement activation (RCA) inhibit complement-induced immune responses on healthy host tissues. We present crystal structures of human RCA (MCP, DAF, and CR1) and a smallpox virus homolog (SPICE) bound to complement component C3b. Our structural data reveal that up to four consecutive homologous CCP domains (i-iv), responsible for inhibition, bind in the same orientation and extended arrangement at a shared binding platform on C3b. Large sequence variations in CCP domains explain the diverse C3b-binding patterns, with limited or no contribution of some individual domains, while all regulators show extensive contacts with C3b for the domains at the third site. A variation of ~100° rotation around the longitudinal axis is observed for domains binding at the fourth site on C3b, without affecting the overall binding mode. The data suggest a common evolutionary origin for both inhibitory mechanisms, called decay acceleration and cofactor activity, with variable C3b binding through domains at sites ii, iii, and iv, and provide a framework for understanding RCA disease-related mutations and immune evasion.

PubMed: 27013439
DOI: 10.15252/EMBJ.201593673

実験手法

X-RAY DIFFRACTION (3.3 Å)