5FN1

Electron cryo-microscopy of filamentous flexible virus PepMV (Pepino Mosaic Virus)

5FN1 の概要

• エントリーDOI: 10.2210/pdb5fn1/pdb
• EMDBエントリー: 3236
• 分子名称: COAT PROTEIN, 5'-R(*UP*UP*UP*UP*UP)-3' (2 entities in total)
• 機能のキーワード: virus, pepmv, filamentous plant virus, potexvirus, helical symmetry
• 由来する生物種: PEPINO MOSAIC VIRUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26632.23

構造登録者

Agirrezabala, X.,Mendez-Lopez, E.,Lasso, G.,Sanchez-Pina, M.A.,Aranda, M.A.,Valle, M. (登録日: 2015-11-10, 公開日: 2015-12-30, 最終更新日: 2024-05-08)

主引用文献

Agirrezabala, X.,Mendez-Lopez, E.,Lasso, G.,Sanchez-Pina, M.A.,Aranda, M.,Valle, M.
The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses.
Elife, 4:e11795-e11795, 2015

PubMed Abstract: Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We describe here a structure at 3.9 Å resolution, from electron cryomicroscopy, of Pepino mosaic virus (PepMV), a representative of the genus Potexvirus (family Alphaflexiviridae). Our results allow modeling of the CP and its interactions with viral RNA. The overall fold of PepMV CP resembles that of nucleoproteins (NPs) from the genus Phlebovirus (family Bunyaviridae), a group of enveloped (-)ssRNA viruses. The main difference between potexvirus CP and phlebovirus NP is in their C-terminal extensions, which appear to determine the characteristics of the distinct multimeric assemblies - a flexuous, helical rod or a loose ribonucleoprotein. The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses.

PubMed: 26673077
DOI: 10.7554/eLife.11795

実験手法

ELECTRON MICROSCOPY (3.9 Å)