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5FMO

Crystal structure and proteomics analysis of empty virus like particles of Cowpea mosaic virus

5FMO の概要
エントリーDOI10.2210/pdb5fmo/pdb
分子名称EMPTY VIRUS LIKE PARTICLES OF COWPEA MOSAIC VIRUS (3 entities in total)
機能のキーワードvirus, cpmv, evlps
由来する生物種COWPEA MOSAIC VIRUS (CPMV)
詳細
タンパク質・核酸の鎖数2
化学式量合計65026.75
構造登録者
Huynh, N.,Hesketh, E.L.,Saxena, P.,Meshcheriakova, Y.,Ku, Y.C.,Hoang, L.,Johnson, J.E.,Ranson, N.A.,Lomonossoff, G.P.,Reddy, V.S. (登録日: 2015-11-07, 公開日: 2016-03-09, 最終更新日: 2024-01-10)
主引用文献Huynh, N.T.,Hesketh, E.L.,Saxena, P.,Meshcheriakova, Y.,Ku, Y.,Hoang, L.T.,Johnson, J.E.,Ranson, N.A.,Lomonossoff, G.P.,Reddy, V.S.
Crystal Structure and Proteomics Analysis of Empty Virus-Like Particles of Cowpea Mosaic Virus.
Structure, 24:567-, 2016
Cited by
PubMed Abstract: Empty virus-like particles (eVLPs) of Cowpea mosaic virus (CPMV) are currently being utilized as reagents in various biomedical and nanotechnology applications. Here, we report the crystal structure of CPMV eVLPs determined using X-ray crystallography at 2.3 Å resolution and compare it with previously reported cryo-electron microscopy (cryo-EM) of eVLPs and virion crystal structures. Although the X-ray and cryo-EM structures of eVLPs are mostly similar, there exist significant differences at the C terminus of the small (S) subunit. The intact C terminus of the S subunit plays a critical role in enabling the efficient assembly of CPMV virions and eVLPs, but undergoes proteolysis after particle formation. In addition, we report the results of mass spectrometry-based proteomics analysis of coat protein subunits from CPMV eVLPs and virions that identify the C termini of S subunits undergo proteolytic cleavages at multiple sites instead of a single cleavage site as previously observed.
PubMed: 27021160
DOI: 10.1016/J.STR.2016.02.011
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 5fmo
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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