5FLZ

Cryo-EM structure of gamma-TuSC oligomers in a closed conformation

5FLZ の概要

• エントリーDOI: 10.2210/pdb5flz/pdb
• 関連するPDBエントリー: 5FM1
• EMDBエントリー: 2799
• 分子名称: SPINDLE POLE BODY COMPONENT SPC97, SPINDLE POLE BODY COMPONENT SPC98, TUBULIN GAMMA CHAIN, ... (4 entities in total)
• 機能のキーワード: cell cycle, microtubule nucleation
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Nucleus: P38863 P53540; Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body: P53378
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 308268.74

構造登録者

Greenberg, C.H.,Kollman, J.,Zelter, A.,Johnson, R.,MacCoss, M.J.,Davis, T.N.,Agard, D.A.,Sali, A. (登録日: 2015-10-29, 公開日: 2016-01-13, 最終更新日: 2024-05-08)

主引用文献

Greenberg, C.H.,Kollman, J.,Zelter, A.,Johnson, R.,Maccoss, M.J.,Davis, T.N.,Agard, D.A.,Sali, A.
Structure of Gamma-Tubulin Small Complex Based on a Cryo-Em Map, Chemical Cross-Links, and a Remotely Related Structure.
J.Struct.Biol., 194:303-, 2016

PubMed Abstract: Modeling protein complex structures based on distantly related homologues can be challenging due to poor sequence and structure conservation. Therefore, utilizing even low-resolution experimental data can significantly increase model precision and accuracy. Here, we present models of the two key functional states of the yeast γ-tubulin small complex (γTuSC): one for the low-activity "open" state and another for the higher-activity "closed" state. Both models were computed based on remotely related template structures and cryo-EM density maps at 6.9Å and 8.0Å resolution, respectively. For each state, extensive sampling of alignments and conformations was guided by the fit to the corresponding cryo-EM density map. The resulting good-scoring models formed a tightly clustered ensemble of conformations in most regions. We found significant structural differences between the two states, primarily in the γ-tubulin subunit regions where the microtubule binds. We also report a set of chemical cross-links that were found to be consistent with equilibrium between the open and closed states. The protocols developed here have been incorporated into our open-source Integrative Modeling Platform (IMP) software package (http://integrativemodeling.org), and can therefore be applied to many other systems.

PubMed: 26968363
DOI: 10.1016/J.JSB.2016.03.006

実験手法

ELECTRON MICROSCOPY (6.9 Å)