5FLY

The FhuD protein from S.pseudintermedius

5FLY の概要

• エントリーDOI: 10.2210/pdb5fly/pdb
• 分子名称: Ferrichrome ABC transporter substrate-binding protein, CADMIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: metal transport, staphylococcal disease, siderophore, transport, iron, class iii solute binding protein
• 由来する生物種: Staphylococcus pseudintermedius
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66003.78

構造登録者

Malito, E. (登録日: 2015-10-29, 公開日: 2015-12-02, 最終更新日: 2024-01-10)

主引用文献

Abate, F.,Cozzi, R.,Maritan, M.,Lo Surdo, P.,Maione, D.,Malito, E.,Bottomley, M.J.
Crystal Structure of Fhud at 1.6 Angstrom Resolution: A Ferrichrome-Binding Protein from the Animal and Human Pathogen Staphylococcus Pseudintermedius
Acta Crystallogr.,Sect.F, 72:214-, 2016

PubMed Abstract: Staphylococcus pseudintermedius is a leading cause of disease in dogs, and zoonosis causes human infections. Methicillin-resistant S. pseudintermedius strains are emerging, resembling the global health threat of S. aureus. Therefore, it is increasingly important to characterize potential targets for intervention against S. pseudintermedius. Here, FhuD, an S. pseudintermedius surface lipoprotein implicated in iron uptake, was characterized. It was found that FhuD bound ferrichrome in an iron-dependent manner, which increased the thermostability of FhuD by >15 °C. The crystal structure of ferrichrome-free FhuD was determined via molecular replacement at 1.6 Å resolution. FhuD exhibits the class III solute-binding protein (SBP) fold, with a ligand-binding cavity between the N- and C-terminal lobes, which is here occupied by a PEG molecule. The two lobes of FhuD were oriented in a closed conformation. These results provide the first detailed structural characterization of FhuD, a potential therapeutic target of S. pseudintermedius.

PubMed: 26919525
DOI: 10.1107/S2053230X16002272

実験手法

X-RAY DIFFRACTION (1.598 Å)