5FLW

Crystal structure of putative exo-beta-1,3-galactanase from Bifidobacterium bifidum s17

5FLW の概要

• エントリーDOI: 10.2210/pdb5flw/pdb
• 分子名称: EXO-BETA-1,3-GALACTANASE, 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: galactanase, bifidobacterium, gh43, family 43, hydrolase
• 由来する生物種: BIFIDOBACTERIUM BIFIDUM
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68299.95

構造登録者

Godoy, A.S.,de Lima, M.Z.T.,Ramia, M.P.,Camilo, C.M.,Muniz, J.R.C.,Polikarpov, I. (登録日: 2015-10-28, 公開日: 2015-12-23, 最終更新日: 2024-01-10)

主引用文献

Godoy, A.S.,de Lima, M.Z.,Camilo, C.M.,Polikarpov, I.
Crystal structure of a putative exo-beta-1,3-galactanase from Bifidobacterium bifidum S17.
Acta Crystallogr F Struct Biol Commun, 72:288-293, 2016

PubMed Abstract: Given the current interest in second-generation biofuels, carbohydrate-active enzymes have become the most important tool to overcome the structural recalcitrance of the plant cell wall. While some glycoside hydrolase families have been exhaustively described, others remain poorly characterized, especially with regard to structural information. The family 43 glycoside hydrolases are a diverse group of inverting enzymes; the available structure information on these enzymes is mainly from xylosidases and arabinofuranosidase. Currently, only one structure of an exo-β-1,3-galactanase is available. Here, the production, crystallization and structure determination of a putative exo-β-1,3-galactanase from Bifidobacterium bifidum S17 (BbGal43A) are described. BbGal43A was successfully produced and showed activity towards synthetic galactosides. BbGal43A was subsequently crystallized and data were collected to 1.4 Å resolution. The structure shows a single-domain molecule, differing from known homologues, and crystal contact analysis predicts the formation of a dimer in solution. Further biochemical studies are necessary to elucidate the differences between BbGal43A and its characterized homologues.

PubMed: 27050262
DOI: 10.1107/S2053230X16003617

実験手法

X-RAY DIFFRACTION (1.401 Å)