5FL2

Revisited cryo-EM structure of Inducible lysine decarboxylase complexed with LARA domain of RavA ATPase

5FL2 の概要

• エントリーDOI: 10.2210/pdb5fl2/pdb
• 関連するPDBエントリー: 5FKX 5FKZ
• EMDBエントリー: 3206
• 分子名称: LYSINE DECARBOXYLASE, INDUCIBLE, ATPASE RAVA (2 entities in total)
• 機能のキーワード: hydrolase-isomerase complex, acid-stress, atpase, rava, hydrolase/isomerase
• 由来する生物種: ESCHERICHIA COLI K-12; 詳細
• 細胞内の位置: Cytoplasm : P0A9H3 P31473
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92544.92

構造登録者

Kandiah, E.,Carriel, D.,Perard, J.,Malet, H.,Bacia, M.,Liu, K.,Chan, S.W.S.,Houry, W.A.,Ollagnier de Choudens, S.,Elsen, S.,Gutsche, I. (登録日: 2015-10-21, 公開日: 2016-09-21, 最終更新日: 2024-05-08)

主引用文献

Kandiah, E.,Carriel, D.,Perard, J.,Malet, H.,Bacia, M.,Liu, K.,Chan, S.W.S.,Houry, W.A.,Ollagnier De Choudens, S.,Elsen, S.,Gutsche, I.
Structural Insights Into the Escherichia Coli Lysine Decarboxylases and Molecular Determinants of Interaction with the Aaa+ ATPase Rava.
Sci.Rep., 6:24601-, 2016

PubMed Abstract: The inducible lysine decarboxylase LdcI is an important enterobacterial acid stress response enzyme whereas LdcC is its close paralogue thought to play mainly a metabolic role. A unique macromolecular cage formed by two decamers of the Escherichia coli LdcI and five hexamers of the AAA+ ATPase RavA was shown to counteract acid stress under starvation. Previously, we proposed a pseudoatomic model of the LdcI-RavA cage based on its cryo-electron microscopy map and crystal structures of an inactive LdcI decamer and a RavA monomer. We now present cryo-electron microscopy 3D reconstructions of the E. coli LdcI and LdcC, and an improved map of the LdcI bound to the LARA domain of RavA, at pH optimal for their enzymatic activity. Comparison with each other and with available structures uncovers differences between LdcI and LdcC explaining why only the acid stress response enzyme is capable of binding RavA. We identify interdomain movements associated with the pH-dependent enzyme activation and with the RavA binding. Multiple sequence alignment coupled to a phylogenetic analysis reveals that certain enterobacteria exert evolutionary pressure on the lysine decarboxylase towards the cage-like assembly with RavA, implying that this complex may have an important function under particular stress conditions.

PubMed: 27080013
DOI: 10.1038/SREP24601

実験手法

ELECTRON MICROSCOPY (6.2 Å)