5FJE

CU(I)-CSP1 (COPPER STORAGE PROTEIN 1) FROM METHYLOSINUS TRICHOSPORIUM OB3B

「5AJF」から置き換えられました

5FJE の概要

• エントリーDOI: 10.2210/pdb5fje/pdb
• 関連するPDBエントリー: 5FJD
• 分子名称: COPPER STORAGE PROTEIN 1, COPPER (I) ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: copper binding protein, methane oxidation, copper storage, methanotrophs, particulate methane monooxygenase
• 由来する生物種: METHYLOSINUS TRICHOSPORIUM OB3B
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26917.23

構造登録者

Vita, N.,Platsaki, S.,Basle, A.,Allen, S.J.,Paterson, N.G.,Crombie, A.T.,Murrell, J.C.,Waldron, K.J.,Dennison, C. (登録日: 2015-10-07, 公開日: 2015-11-18, 最終更新日: 2024-05-08)

主引用文献

Vita, N.,Platsaki, S.,Basle, A.,Allen, S.J.,Paterson, N.G.,Crombie, A.T.,Murrell, J.C.,Waldron, K.J.,Dennison, C.
A Four-Helix Bundle Stores Copper for Methane Oxidation.
Nature, 525:140-, 2015

PubMed Abstract: Methane-oxidizing bacteria (methanotrophs) require large quantities of copper for the membrane-bound (particulate) methane monooxygenase. Certain methanotrophs are also able to switch to using the iron-containing soluble methane monooxygenase to catalyse methane oxidation, with this switchover regulated by copper. Methane monooxygenases are nature's primary biological mechanism for suppressing atmospheric levels of methane, a potent greenhouse gas. Furthermore, methanotrophs and methane monooxygenases have enormous potential in bioremediation and for biotransformations producing bulk and fine chemicals, and in bioenergy, particularly considering increased methane availability from renewable sources and hydraulic fracturing of shale rock. Here we discover and characterize a novel copper storage protein (Csp1) from the methanotroph Methylosinus trichosporium OB3b that is exported from the cytosol, and stores copper for particulate methane monooxygenase. Csp1 is a tetramer of four-helix bundles with each monomer binding up to 13 Cu(I) ions in a previously unseen manner via mainly Cys residues that point into the core of the bundle. Csp1 is the first example of a protein that stores a metal within an established protein-folding motif. This work provides a detailed insight into how methanotrophs accumulate copper for the oxidation of methane. Understanding this process is essential if the wide-ranging biotechnological applications of methanotrophs are to be realized. Cytosolic homologues of Csp1 are present in diverse bacteria, thus challenging the dogma that such organisms do not use copper in this location.

PubMed: 26308900
DOI: 10.1038/NATURE14854

実験手法

X-RAY DIFFRACTION (1.9 Å)