5FJ9

Cryo-EM structure of yeast apo RNA polymerase III at 4.6 A

5FJ9 の概要

• エントリーDOI: 10.2210/pdb5fj9/pdb
• 関連するPDBエントリー: 5FJ8 5FJA
• EMDBエントリー: 3179
• 分子名称: DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1, DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 5, DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2, ... (18 entities in total)
• 機能のキーワード: rna polymerase iii, pol iii, transcription, rna polymerase
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 677098.68

構造登録者

Hoffmann, N.A.,Jakobi, A.J.,Moreno-Morcillo, M.,Glatt, S.,Kosinski, J.,Hagen, W.J.,Sachse, C.,Muller, C.W. (登録日: 2015-10-06, 公開日: 2015-11-25, 最終更新日: 2024-10-23)

主引用文献

Hoffmann, N.A.,Jakobi, A.J.,Moreno-Morcillo, M.,Glatt, S.,Kosinski, J.,Hagen, W.J.H.,Sachse, C.,Muller, C.W.
Molecular Structures of Unbound and Transcribing RNA Polymerase III.
Nature, 528:231-, 2015

PubMed Abstract: Transcription of genes encoding small structured RNAs such as transfer RNAs, spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. Here we present the cryo-electron microscopy structures of the Saccharomyces cerevisiae Pol III elongating complex at 3.9 Å resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Å resolution, respectively, which allow the building of a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82-C34-C31 heterotrimer in close proximity to the stalk. The C53-C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets.

PubMed: 26605533
DOI: 10.1038/NATURE16143

実験手法

ELECTRON MICROSCOPY (4.6 Å)