5FIY

crystal structure of coiled coil domain of PAWR

5FIY の概要

• エントリーDOI: 10.2210/pdb5fiy/pdb
• 分子名称: PRKC APOPTOSIS WT1 REGULATOR PROTEIN, POTASSIUM ION (3 entities in total)
• 機能のキーワード: apoptosis
• 由来する生物種: RATTUS NORVEGICUS (NORWAY RAT)
• 細胞内の位置: Cytoplasm : Q62627
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 87513.45

構造登録者

Tiruttani Subhramanyam, U.K.,Kubicek, J.,Eidhoff, U.B.,Labahn, J. (登録日: 2015-10-03, 公開日: 2016-11-16, 最終更新日: 2024-11-20)

主引用文献

Tiruttani Subhramanyam, U.K.,Kubicek, J.,Eidhoff, U.B.,Labahn, J.
Structural basis for the regulatory interactions of proapoptotic Par-4.
Cell Death Differ., 24:1540-1547, 2017

PubMed Abstract: Par-4 is a unique proapoptotic protein with the ability to induce apoptosis selectively in cancer cells. The X-ray crystal structure of the C-terminal domain of Par-4 (Par-4), which regulates its apoptotic function, was obtained by MAD phasing. Par-4 homodimerizes by forming a parallel coiled-coil structure. The N-terminal half of Par-4 contains the homodimerization subdomain. This structure includes a nuclear export signal (Par-4) sequence, which is masked upon dimerization indicating a potential mechanism for nuclear localization. The heteromeric-interaction models specifically showed that charge interaction is an important factor in the stability of heteromers of the C-terminal leucine zipper subdomain of Par-4 (Par-4). These heteromer models also displayed NES masking capacity and therefore the ability to influence intracellular localization.

PubMed: 28622290
DOI: 10.1038/cdd.2017.76

実験手法

X-RAY DIFFRACTION (3 Å)