5FII

Structure of a human aspartate kinase, chorismate mutase and TyrA domain.

5FII の概要

• エントリーDOI: 10.2210/pdb5fii/pdb
• 分子名称: PHENYLALANINE-4-HYDROXYLASE, PHENYLALANINE (3 entities in total)
• 機能のキーワード: oxidoreductase, transferase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47473.84

構造登録者

Patel, D.,Kopec, J.,Shrestha, L.,Fitzpatrick, F.,Pinkas, D.,Chaikuad, A.,Dixon-Clarke, S.,McCorvie, T.J.,Burgess-Brown, N.,von Delft, F.,Arrowsmith, C.,Edwards, A.,Bountra, C.,Yue, W.W. (登録日: 2015-09-25, 公開日: 2016-03-30, 最終更新日: 2024-05-08)

主引用文献

Patel, D.,Kopec, J.,Fitzpatrick, F.,Mccorvie, T.J.,Yue, W.W.
Structural Basis for Ligand-Dependent Dimerization of Phenylalanine Hydroxylase Regulatory Domain.
Sci.Rep., 6:23748-, 2016

PubMed Abstract: The multi-domain enzyme phenylalanine hydroxylase (PAH) catalyzes the hydroxylation of dietary I-phenylalanine (Phe) to I-tyrosine. Inherited mutations that result in PAH enzyme deficiency are the genetic cause of the autosomal recessive disorder phenylketonuria. Phe is the substrate for the PAH active site, but also an allosteric ligand that increases enzyme activity. Phe has been proposed to bind, in addition to the catalytic domain, a site at the PAH N-terminal regulatory domain (PAH-RD), to activate the enzyme via an unclear mechanism. Here we report the crystal structure of human PAH-RD bound with Phe at 1.8 Å resolution, revealing a homodimer of ACT folds with Phe bound at the dimer interface. This work delivers the structural evidence to support previous solution studies that a binding site exists in the RD for Phe, and that Phe binding results in dimerization of PAH-RD. Consistent with our structural observation, a disease-associated PAH mutant impaired in Phe binding disrupts the monomer:dimer equilibrium of PAH-RD. Our data therefore support an emerging model of PAH allosteric regulation, whereby Phe binds to PAH-RD and mediates the dimerization of regulatory modules that would bring about conformational changes to activate the enzyme.

PubMed: 27049649
DOI: 10.1038/SREP23748

実験手法

X-RAY DIFFRACTION (1.8 Å)