5FH2

The structure of rat cytosolic PEPCK variant E89Q in complex with GTP

5FH2 の概要

• エントリーDOI: 10.2210/pdb5fh2/pdb
• 関連するPDBエントリー: 5FH0 5FH1 5FH3 5FH4 5FH5
• 分子名称: Phosphoenolpyruvate carboxykinase, cytosolic [GTP], MANGANESE (II) ION, GUANOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: kinase, gluconeogenesis, lyase
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 70393.03

構造登録者

Johnson, T.A.,Holyoak, T. (登録日: 2015-12-21, 公開日: 2016-11-09, 最終更新日: 2023-09-27)

主引用文献

Johnson, T.A.,Mcleod, M.J.,Holyoak, T.
Utilization of Substrate Intrinsic Binding Energy for Conformational Change and Catalytic Function in Phosphoenolpyruvate Carboxykinase.
Biochemistry, 55:575-587, 2016

PubMed Abstract: Phosphoenolpyruvate carboxykinase (PEPCK) is an essential metabolic enzyme operating in the gluconeogenesis and glyceroneogenesis pathways. Previous work has demonstrated that the enzyme cycles between a catalytically inactive open state and a catalytically active closed state. The transition of the enzyme between these states requires the transition of several active site loops to shift from mobile, disordered structural elements to stable ordered states. The mechanism by which these disorder-order transitions are coupled to the ligation state of the active site however is not fully understood. To further investigate the mechanisms by which the mobility of the active site loops is coupled to enzymatic function and the transitioning of the enzyme between the two conformational states, we have conducted structural and functional studies of point mutants of E89. E89 is a proposed key member of the interaction network of mobile elements as it resides in the R-loop region of the enzyme active site. These new data demonstrate the importance of the R-loop in coordinating interactions between substrates at the OAA/PEP binding site and the mobile R- and Ω-loop domains. In turn, the studies more generally demonstrate the mechanisms by which the intrinsic ligand binding energy can be utilized in catalysis to drive unfavorable conformational changes, changes that are subsequently required for both optimal catalytic activity and fidelity.

PubMed: 26709450
DOI: 10.1021/acs.biochem.5b01215

実験手法

X-RAY DIFFRACTION (1.49 Å)