5FD9
X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation B)
Summary for 5FD9
| Entry DOI | 10.2210/pdb5fd9/pdb |
| Related | 5FD7 |
| Descriptor | Vacuolar-sorting protein SNF7 (2 entities in total) |
| Functional Keywords | escrt, snf7, active, core, protein transport |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Total number of polymer chains | 1 |
| Total formula weight | 15803.13 |
| Authors | Tang, S.,Henne, W.M.,Borbat, P.P.,Buchkovich, N.J.,Freed, J.H.,Mao, Y.,Fromme, J.C.,Emr, S.D. (deposition date: 2015-12-15, release date: 2015-12-30, Last modification date: 2023-09-27) |
| Primary citation | Tang, S.,Henne, W.M.,Borbat, P.P.,Buchkovich, N.J.,Freed, J.H.,Mao, Y.,Fromme, J.C.,Emr, S.D. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. Elife, 4:-, 2015 Cited by PubMed Abstract: The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes. Although ESCRT-III subunits polymerize into spirals, how individual ESCRT-III subunits are activated and assembled together into a membrane-deforming filament remains unknown. Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation. Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. This promotes the assembly of Snf7 arrays with ~30 Å periodicity into a membrane-sculpting filament. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block ESCRT function. The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling. PubMed: 26670543DOI: 10.7554/eLife.12548 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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