5FA9

Bifunctional Methionine Sulfoxide Reductase AB (MsrAB) from Treponema denticola

Summary for 5FA9

• Entry DOI: 10.2210/pdb5fa9/pdb
• Descriptor: Peptide methionine sulfoxide reductase MsrA, (4S,5S)-1,2-DITHIANE-4,5-DIOL, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, ... (4 entities in total)
• Functional Keywords: methionine sulfoxide reductase(msr), fusion protein, protein oxidation, oxidoreductase
• Biological source: Treponema denticola ATCC 35405
• Total number of polymer chains: 2
• Total formula weight: 74890.69

Authors

Han, A.,Son, J.,Kim, H.-Y.,Hwang, K.Y. (deposition date: 2015-12-11, release date: 2016-09-14, Last modification date: 2023-11-08)

Primary citation

Han, A.R.,Kim, M.J.,Kwak, G.H.,Son, J.,Hwang, K.Y.,Kim, H.Y.
Essential Role of the Linker Region in the Higher Catalytic Efficiency of a Bifunctional MsrA-MsrB Fusion Protein
Biochemistry, 55:5117-5127, 2016

PubMed Abstract: Many bacteria, particularly pathogens, possess methionine sulfoxide reductase A (MsrA) and B (MsrB) as a fusion form (MsrAB). However, it is not clear why they possess a fusion MsrAB form rather than the separate enzymes that exist in most organisms. In this study, we performed biochemical and kinetic analyses of MsrAB from Treponema denticola (TdMsrAB), single-domain forms (TdMsrA and TdMsrB), and catalytic Cys mutants (TdMsrAB(C11S) and TdMsrAB(C285S)). We found that the catalytic efficiency of both MsrA and MsrB increased after fusion of the domains and that the linker region (iloop) that connects TdMsrA and TdMsrB is required for the higher catalytic efficiency of TdMsrAB. We also determined the crystal structure of TdMsrAB at 2.3 Å, showing that the iloop mainly interacts with TdMsrB via hydrogen bonds. Further kinetic analysis using the iloop mutants revealed that the iloop-TdMsrB interactions are critical to MsrB and MsrA activities. We also report the structure in which an oxidized form of dithiothreitol, an in vitro reductant for MsrA and MsrB, is present in the active site of TdMsrA. Collectively, the results of this study reveal an essential role of the iloop in maintaining the higher catalytic efficiency of the MsrAB fusion enzyme and provide a better understanding of why the MsrAB enzyme exists as a fused form.

PubMed: 27551953
DOI: 10.1021/acs.biochem.6b00544

Experimental method

X-RAY DIFFRACTION (2.302 Å)