5F9K

Dictyostelium discoideum dUTPase at 2.2 Angstrom

Summary for 5F9K

Entry DOI	10.2210/pdb5f9k/pdb
Descriptor	Deoxyuridine 5'-triphosphate nucleotidohydrolase, 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE, MAGNESIUM ION, ... (5 entities in total)
Functional Keywords	hydrolase
Biological source	Dictyostelium discoideum (Slime mold)
Total number of polymer chains	3
Total formula weight	48243.76
Authors	Inoguchi, N.,Chia, C.P.,Heck, K.,Moriyama, H. (deposition date: 2015-12-09, release date: 2016-12-14, Last modification date: 2023-09-27)
Primary citation	Chia, C.P.,Inoguchi, N.,Varon, K.C.,Bartholomai, B.M.,Moriyama, H. Mitochondrial localization of Dictyostelium discoideum dUTPase mediated by its N-terminus. Bmc Res Notes, 13:16-16, 2020 Cited by PubMed Abstract: The nuclear and mitochondrial genomes of Dictyostelium discoideum, a unicellular eukaryote, have relatively high A+T-contents of 77.5% and 72.65%, respectively. To begin to investigate how the pyrimidine biosynthetic pathway fulfills the demand for dTTP, we determined the catalytic properties and structure of the key enzyme deoxyuridine triphosphate nucleotidohydrolase (dUTPase) that hydrolyzes dUTP to dUMP, the precursor of dTTP. PubMed: 31910901 DOI: 10.1186/s13104-019-4879-7 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.179 Å)

Structure validation