5F8Y

Crystal structure of a Crenomytilus grayanus lectin in complex with galactosamine

5F8Y の概要

• エントリーDOI: 10.2210/pdb5f8y/pdb
• 関連するPDBエントリー: 5F8S 5F8W 5F90
• 分子名称: GalNAc/Gal-specific lectin, 2-amino-2-deoxy-alpha-D-galactopyranose, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: lectin, sugar binding protein
• 由来する生物種: Crenomytilus grayanus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36931.64

構造登録者

Liao, J.-H.,Huang, K.-F.,Tu, I.-F.,Lee, I.-M.,Wu, S.-H. (登録日: 2015-12-09, 公開日: 2016-04-06, 最終更新日: 2024-03-20)

主引用文献

Liao, J.-H.,Chien, C.-T.,Wu, H.-Y.,Huang, K.-F.,Wang, I.,Ho, M.-R.,Tu, I.-F.,Lee, I.-M.,Li, W.,Shih, Y.-L.,Wu, C.-Y.,Lukyanov, P.A.,Hsu, S.D.,Wu, S.-H.
A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3
J.Am.Chem.Soc., 138:4787-4795, 2016

PubMed Abstract: In this study, we report the structure and function of a lectin from the sea mollusk Crenomytilus grayanus collected from the sublittoral zone of Peter the Great Bay of the Sea of Japan. The crystal structure of C. grayanus lectin (CGL) was solved to a resolution of 1.08 Å, revealing a β-trefoil fold that dimerizes into a dumbbell-shaped quaternary structure. Analysis of the crystal CGL structures bound to galactose, galactosamine, and globotriose Gb3 indicated that each CGL can bind three ligands through a carbohydrate-binding motif involving an extensive histidine- and water-mediated hydrogen bond network. CGL binding to Gb3 is further enhanced by additional side-chain-mediated hydrogen bonds in each of the three ligand-binding sites. NMR titrations revealed that the three binding sites have distinct microscopic affinities toward galactose and galactosamine. Cell viability assays showed that CGL recognizes Gb3 on the surface of breast cancer cells, leading to cell death. Our findings suggest the use of this lectin in cancer diagnosis and treatment.

PubMed: 27010847
DOI: 10.1021/jacs.6b00111

実験手法

X-RAY DIFFRACTION (1.7 Å)