5F81

Acoustic injectors for drop-on-demand serial femtosecond crystallography

5F81 の概要

• エントリーDOI: 10.2210/pdb5f81/pdb
• 関連するPDBエントリー: 5F80
• 分子名称: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: lysozyme, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14531.41

構造登録者

Roessler, C.G.,Agarwal, R.,Allaire, M.,Alonso-Mori, R.,Andi, B.,Bachega, J.F.R.,Bommer, M.,Brewster, A.S.,Browne, M.C.,Chatterjee, R.,Cho, E.,Cohen, A.E.,Cowan, M.,Datwani, S.,Davidson, V.L.,Defever, J.,Eaton, B.,Ellson, R.,Feng, Y.,Ghislain, L.P.,Glownia, J.M.,Han, G.,Hattne, J.,Hellmich, J.,Heroux, A.,Ibrahim, M.,Kern, J.,Kuczewski, A.,Lemke, H.T.,Liu, P.,Majlof, L.,McClintock, W.M.,Myers, S.,Nelsen, S.,Olechno, J.,Orville, A.M.,Sauter, N.K.,Soares, A.S.,Soltis, M.S.,Song, H.,Stearns, R.G.,Tran, R.,Tsai, Y.,Uervirojnangkoorn, M.,Wilmot, C.M.,Yachandra, V.,Yano, J.,Yukl, E.T.,Zhu, D.,Zouni, A. (登録日: 2015-12-08, 公開日: 2016-02-10, 最終更新日: 2024-01-10)

主引用文献

Roessler, C.G.,Agarwal, R.,Allaire, M.,Alonso-Mori, R.,Andi, B.,Bachega, J.F.,Bommer, M.,Brewster, A.S.,Browne, M.C.,Chatterjee, R.,Cho, E.,Cohen, A.E.,Cowan, M.,Datwani, S.,Davidson, V.L.,Defever, J.,Eaton, B.,Ellson, R.,Feng, Y.,Ghislain, L.P.,Glownia, J.M.,Han, G.,Hattne, J.,Hellmich, J.,Heroux, A.,Ibrahim, M.,Kern, J.,Kuczewski, A.,Lemke, H.T.,Liu, P.,Majlof, L.,McClintock, W.M.,Myers, S.,Nelsen, S.,Olechno, J.,Orville, A.M.,Sauter, N.K.,Soares, A.S.,Soltis, S.M.,Song, H.,Stearns, R.G.,Tran, R.,Tsai, Y.,Uervirojnangkoorn, M.,Wilmot, C.M.,Yachandra, V.,Yano, J.,Yukl, E.T.,Zhu, D.,Zouni, A.
Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.
Structure, 24:631-640, 2016

PubMed Abstract: X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

PubMed: 26996959
DOI: 10.1016/j.str.2016.02.007

実験手法

X-RAY DIFFRACTION (2.13 Å)