5F3Y

Crystal Structure of Myo7b N-MyTH4-FERM-SH3 in complex with Anks4b CEN

5F3Y の概要

• エントリーDOI: 10.2210/pdb5f3y/pdb
• 分子名称: Unconventional myosin-VIIb, Ankyrin repeat and SAM domain-containing protein 4B (2 entities in total)
• 機能のキーワード: motor protein, protein binding, structural protein, protein transport, motor protein-protein binding complex, motor protein/protein binding
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Apical cell membrane : Q99MZ6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81598.24

構造登録者

Li, J.,He, Y.,Lu, Q.,Zhang, M. (登録日: 2015-12-03, 公開日: 2016-03-16, 最終更新日: 2023-11-08)

主引用文献

Li, J.,He, Y.,Lu, Q.,Zhang, M.
Mechanistic Basis of Organization of the Harmonin/USH1C-Mediated Brush Border Microvilli Tip-Link Complex
Dev.Cell, 36:179-189, 2016

PubMed Abstract: Brush border microvilli are actin-based protrusions lining the apical surface of epithelial cells in intestines and proximal tubules of kidneys. While brush border microvilli resemble the relatively well-characterized stereocilia of hair cells, the mechanistic basis of tip-link complex organization in microvilli is poorly understood. Here, we have biochemically and structurally characterized the following pairs of interactions: protocadherin 24 and Harmonin (also known as USH1C or AIE-75), Harmonin and myosin VIIb (MYO7B), Harmonin and ANKS4B, and ANKS4B and MYO7B. We show that Harmonin, ANKS4B, and MYO7B form a stable ternary complex for anchoring microvilli tip-link cadherins. Despite having only Harmonin in common, the microvilli and the stereocilia tip-link complexes are formed via strikingly similar interaction modes. These results not only provide insight into the mechanistic bases of brush border microvilli formation and maintenance but may also be valuable for understanding some gut and/or kidney diseases caused by perturbations of brush border microvilli structures.

PubMed: 26812017
DOI: 10.1016/j.devcel.2015.12.020

実験手法

X-RAY DIFFRACTION (3.409 Å)