5F14

Structure of native hen egg-white lysozyme

Summary for 5F14

• Entry DOI: 10.2210/pdb5f14/pdb
• Related: 1IEE 5F16
• Descriptor: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: lysozyme, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14460.51

Authors

McGlone, C.,Nix, J.C.,Page, R.C. (deposition date: 2015-11-30, release date: 2016-02-24, Last modification date: 2024-10-23)

Primary citation

Lucius, M.,Falatach, R.,McGlone, C.,Makaroff, K.,Danielson, A.,Williams, C.,Nix, J.C.,Konkolewicz, D.,Page, R.C.,Berberich, J.A.
Investigating the Impact of Polymer Functional Groups on the Stability and Activity of Lysozyme-Polymer Conjugates.
Biomacromolecules, 17:1123-1134, 2016

PubMed Abstract: Polymers are often conjugated to proteins to improve stability; however, the impact of polymer chain length and functional groups on protein structure and function is not well understood. Here we use RAFT polymerization to grow polymers of different lengths and functionality from a short acrylamide oligomer with a RAFT end group conjugated to lysozyme. We show by X-ray crystallography that enzyme structure is minimally impacted by modification with the RAFT end group. Significant activity toward the negatively charged Micrococcus lysodeicticus cell wall was maintained when lysozyme was modified with cationic polymers. Thermal and chemical stability of the conjugates was characterized using differential scanning fluorimetry and tryptophan fluorescence. All conjugates had a lower melting temperature; however, conjugates containing ionic or substrate mimicking polymers were more resistant to denaturation by guanidine hydrochloride. Our results demonstrate that tailoring polymer functionality can improve conjugate activity and minimize enzymatic inactivation by denaturants.

PubMed: 26866284
DOI: 10.1021/acs.biomac.5b01743

Experimental method

X-RAY DIFFRACTION (1.148 Å)