5F0N

Cohesin subunit Pds5

5F0N の概要

• エントリーDOI: 10.2210/pdb5f0n/pdb
• 分子名称: cohesin subunit Pds5,cohesin subunit Pds5, cohesin subunit Pds5,KLTH0D07062p,cohesin subunit Pds5,cohesin subunit Pds5, cohesin subunit Pds5,KLTH0D07062p,cohesin subunit Pds5,cohesin subunit Pds5, cohesin subunit Pds5,KLTH0D07062p,cohesin subunit Pds5,cohesin subunit Pds5, cohesin subunit Pds5 (1 entity in total)
• 機能のキーワード: heat repeat cohesin subunit, cell cycle
• 由来する生物種: Lachancea thermotolerans (Yeast); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 129680.06

構造登録者

Lee, B.-G.,Jansma, M.,Nasmyth, K.,Lowe, J. (登録日: 2015-11-27, 公開日: 2016-04-20, 最終更新日: 2024-11-20)

主引用文献

Lee, B.G.,Roig, M.B.,Jansma, M.,Petela, N.,Metson, J.,Nasmyth, K.,Lowe, J.
Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1.
Cell Rep, 14:2108-2115, 2016

PubMed Abstract: Sister chromatid cohesion is mediated by cohesin, whose Smc1, Smc3, and kleisin (Scc1) subunits form a ring structure that entraps sister DNAs. The ring is opened either by separase, which cleaves Scc1 during anaphase, or by a releasing activity involving Wapl, Scc3, and Pds5, which bind to Scc1 and open its interface with Smc3. We present crystal structures of Pds5 from the yeast L. thermotolerans in the presence and absence of the conserved Scc1 region that interacts with Pds5. Scc1 binds along the spine of the Pds5 HEAT repeat fold and is wedged between the spine and C-terminal hook of Pds5. We have isolated mutants that confirm the observed binding mode of Scc1 and verified their effect on cohesin by immunoprecipitation and calibrated ChIP-seq. The Pds5 structure also reveals architectural similarities to Scc3, the other large HEAT repeat protein of cohesin and, most likely, Scc2.

PubMed: 26923598
DOI: 10.1016/j.celrep.2016.02.020

実験手法

X-RAY DIFFRACTION (3.2 Å)