5EZ2

Sandercyanin Fluorescent Protein (SFP)

5EZ2 の概要

• エントリーDOI: 10.2210/pdb5ez2/pdb
• 関連するPDBエントリー: 5F1E
• 分子名称: Sandercyanin Fluorescent Protein, BILIVERDINE IX ALPHA, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: sandercyanin, red-fluorescent protein, lipocalin, biliverdin, photo-stability, fluorescent protein
• 由来する生物種: Sander vitreus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41795.13

構造登録者

Ghosh, S.,Ramaswamy, S. (登録日: 2015-11-26, 公開日: 2016-09-28, 最終更新日: 2024-11-20)

主引用文献

Ghosh, S.,Yu, C.L.,Ferraro, D.J.,Sudha, S.,Pal, S.K.,Schaefer, W.F.,Gibson, D.T.,Ramaswamy, S.
Blue protein with red fluorescence
Proc.Natl.Acad.Sci.USA, 113:11513-11518, 2016

PubMed Abstract: The walleye (Sander vitreus) is a golden yellow fish that inhabits the Northern American lakes. The recent sightings of the blue walleye and the correlation of its sighting to possible increased UV radiation have been proposed earlier. The underlying molecular basis of its adaptation to increased UV radiation is the presence of a protein (Sandercyanin)-ligand complex in the mucus of walleyes. Degradation of heme by UV radiation results in the formation of Biliverdin IXα (BLA), the chromophore bound to Sandercyanin. We show that Sandercyanin is a monomeric protein that forms stable homotetramers on addition of BLA to the protein. A structure of the Sandercyanin-BLA complex, purified from the fish mucus, reveals a glycosylated protein with a lipocalin fold. This protein-ligand complex absorbs light in the UV region (λ of 375 nm) and upon excitation at this wavelength emits in the red region (λ of 675 nm). Unlike all other known biliverdin-bound fluorescent proteins, the chromophore is noncovalently bound to the protein. We provide here a molecular rationale for the observed spectral properties of Sandercyanin.

PubMed: 27688756
DOI: 10.1073/pnas.1525622113

実験手法

X-RAY DIFFRACTION (1.849 Å)