5EYY

Tetragonal Form of Centrolobium tomentosum seed lectin (CTL) complexed with Man1-3Man-OMe.

5EYY の概要

• エントリーDOI: 10.2210/pdb5eyy/pdb
• 関連するPDBエントリー: 5EXY
• 分子名称: Centrolobium tomentosum lectin, alpha-D-mannopyranose-(1-3)-methyl alpha-D-mannopyranoside, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: lectin, centrolobium tomentosum, dalbergieae, ctl, methyl dimannoside., sugar binding protein
• 由来する生物種: Centrolobium tomentosum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27754.36

構造登録者

Pinto-Junior, V.R.,Osterne, V.J.S.,Santiago, M.Q.,Almeida, A.C.,Lossio, C.F.,Silva-Filho, J.C.,Almeida, R.P.H.,Teixeira, C.S.,Delatorre, P.,Rocha, B.A.M.,Nascimento, K.S.,Cavada, B.S. (登録日: 2015-11-25, 公開日: 2016-03-16, 最終更新日: 2024-10-23)

主引用文献

Almeida, A.C.,Osterne, V.J.,Santiago, M.Q.,Pinto-Junior, V.R.,Silva-Filho, J.C.,Lossio, C.F.,Nascimento, F.L.,Almeida, R.P.,Teixeira, C.S.,Leal, R.B.,Delatorre, P.,Rocha, B.A.,Assreuy, A.M.,Nascimento, K.S.,Cavada, B.S.
Structural analysis of Centrolobium tomentosum seed lectin with inflammatory activity.
Arch.Biochem.Biophys., 596:73-83, 2016

PubMed Abstract: A glycosylated lectin (CTL) with specificity for mannose and glucose has been detected and purified from seeds of Centrolobium tomentosum, a legume plant from Dalbergieae tribe. It was isolated by mannose-sepharose affinity chromatography. The primary structure was determined by tandem mass spectrometry and consists of 245 amino acids, similar to other Dalbergieae lectins. CTL structures were solved from two crystal forms, a monoclinic and a tetragonal, diffracted at 2.25 and 1.9 Å, respectively. The carbohydrate recognition domain (CRD), metal-binding site and glycosylation site were characterized, and the structural basis for mannose/glucose-binding was elucidated. The lectin adopts the canonical dimeric organization of legume lectins. CTL showed acute inflammatory effect in paw edema model. The protein was subjected to ligand screening (dimannosides and trimannoside) by molecular docking, and interactions were compared with similar lectins possessing the same ligand specificity. This is the first crystal structure of mannose/glucose native seed lectin with proinflammatory activity isolated from the Centrolobium genus.

PubMed: 26946944
DOI: 10.1016/j.abb.2016.03.001

実験手法

X-RAY DIFFRACTION (1.902 Å)